Departamento de Radiologia e Oncologia - FM/MDR
URI Permanente desta comunidade
O Departamento de Radiologia e Oncologia da Faculdade de Medicina da USP (FMUSP) é responsável pelos programas de graduação e pós-graduação nas áreas de Oncologia e Radiologia, oferecendo programas de Mestrado (Oncologia) e Doutorado (Oncologia e Radiologia).Como apoio às atividades de ensino e pesquisa o Departamento dispõe de uma estrutura que comporta uma Biblioteca especializada em Radiologia e Medicina Nuclear, Secretarias de Graduação e Pós-Graduação, um Centro de Pesquisa Clínica e Laboratórios de Investigação Médica em Oncologia Experimental, Medicina Nuclear e Ressonância Magnética em Neurorradiologia.
Site oficial: http://inrad.hc.fm.usp.br/
Site oficial: http://inrad.hc.fm.usp.br/
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- Departamento de Radiologia - FM/MDR
- Departamento de Radiologia - FM/MDR
- Departamento de Radiologia - FM/MDR
Submissões Recentes
Saúde cardiovascular e habitação: um diálogo importante travado nos assentamentos precários de São Paulo
(2023) BARROZO, LIGIA VIZEU; LEITE, CARLOS; AMARO JR., EDSON
ABSTRACT The reduction of infectious diseases and longer life favored the greater prevalence of chronic diseases such as cancer, diabetes, cardiovascular and respiratory diseases. In general, the geographical scale of studies on socioeconomic conditions and health problems is the municipality. In this geographical scale there is no clear separation between the types of settlement. Intraurban studies by type of settlement can contribute to a more faithful portrait on inequalities in living conditions. In this study, data from residents of the municipality of São Paulo were analyzed who were admitted and those who died, by diseases of the circulatory system from 2010 to 2016 for deaths and from 2011 to 2016, for hospitalizations. Each hospitalization and death was attributed to the settlement of housing according to type: non-regular settlement, precarious or regular. The following calculations were made: proportion of hospital admissions by the Unified Health System (SUS) by groups of causes, standardized rates by gender and age group, hospitalization/inhabitant according to sex, age group and type of settlement and standardized rates by gender and age standardized mortality rates due to circulatory diseases. The results found an even more wicked situation in precarious settlements for all age groups, both sexes. The difference in cardiovascular health between the three types of settlements, evaluated through the proportions of hospital hospitalizations and mortality rates, show that almost 1,700,000 people in São Paulo are in a major disadvantage compared to the group formed by 85% of the population. In terms of hospitalizations, rates are higher in non-regular settlement. In this sense, it can be speculated that there is greater access to health service for residents of non-regular settlement in relation to those of precarious settlements. This access is reverted to lower mortality when the rates of both groups are compared. If we consider only two groups (regular and non-regular settlements), the situation of greater disadvantage in precarious settlements is diluted, becoming invisible. Such results can provide health surveillance to define specific programs for these settlements, as well as territory management, in general, to provide better housing conditions.
Epidemiologia do câncer de pulmão: visão do oncologista: ocidente versus oriente
(2022) CASTRO JUNIOR, Gilberto de; MACHADO, Maria Cecília Mathias; YEN, Cheng Tzu
Prefácio
(2023) CERRI, Giovanni Guido
Prefácio
(2023) CERRI, Giovanni Guido
Polymorphisms in transcription factor binding sites and enhancer regions and pancreatic ductal adenocarcinoma risk
(2024) UENAL, Pelin; LU, Ye; BUENO-DE-MESQUITA, Bas; EIJCK, Casper H. J. van; TALAR-WOJNAROWSKA, Renata; SZENTESI, Andrea; GAZOULI, Maria; KREIVENAITE, Edita; TAVANO, Francesca; MALECKA-WOJCIESKO, Ewa; EROSS, Balint; OLIVERIUS, Martin; BUNDUC, Stefania; AOKI, Mateus Nobrega; VODICKOVA, Ludmila; BOGGI, Ugo; GIACCHERINI, Matteo; KONDRACKIENE, Jurate; CHAMMAS, Roger; PALMIERI, Orazio; THEODOROPOULOS, George E.; BIJLSMA, Maarten F.; BASSO, Daniela; MOHELNIKOVA-DUCHONOVA, Beatrice; SOUCEK, Pavel; IZBICKI, Jakob R.; KIUDELIS, Vytautas; VANELLA, Giuseppe; ARCIDIACONO, Paolo Giorgio; WLODARCZYK, Barbara; HACKERT, Thilo; SCHOETTKER, Ben; UZUNOGLU, Faik G.; BAMBI, Franco; GOETZ, Mara; HLAVAC, Viktor; BRENNER, Hermann; PERRI, Francesco; CARRARA, Silvia; LANDI, Stefano; HEGYI, Peter; DIJK, Frederike; MAIELLO, Evaristo; CAPRETTI, Giovanni; TESTONI, Sabrina Gloria Giulia; PETRONE, Maria Chiara; STOCKER, Hannah; ERMINI, Stefano; ARCHIBUGI, Livia; GENTILUOMO, Manuel; CAVESTRO, Giulia Martina; PEZZILLI, Raffaele; FRANCO, Gregorio Di; MILANETTO, Anna Caterina; SPERTI, Cosimo; NEOPTOLEMOS, John P.; MORELLI, Luca; VOKACOVA, Klara; PASQUALI, Claudio; LAWLOR, Rita T.; BAZZOCCHI, Francesca; KUPCINSKAS, Juozas; CAPURSO, Gabriele; CAMPA, Daniele; CANZIAN, Federico
Genome-wide association studies (GWAS) are a powerful tool for detecting variants associated with complex traits and can help risk stratification and prevention strategies against pancreatic ductal adenocarcinoma (PDAC). However, the strict significance threshold commonly used makes it likely that many true risk loci are missed. Functional annotation of GWAS polymorphisms is a proven strategy to identify additional risk loci. We aimed to investigate single-nucleotide polymorphisms (SNP) in regulatory regions [transcription factor binding sites (TFBSs) and enhancers] that could change the expression profile of multiple genes they act upon and thereby modify PDAC risk. We analyzed a total of 12,636 PDAC cases and 43,443 controls from PanScan/PanC4 and the East Asian GWAS (discovery populations), and the PANDoRA consortium (replication population). We identified four associations that reached study-wide statistical significance in the overall meta-analysis: rs2472632(A) (enhancer variant, OR 1.10, 95%CI 1.06,1.13, p = 5.5 x 10-8), rs17358295(G) (enhancer variant, OR 1.16, 95%CI 1.10,1.22, p = 6.1 x 10-7), rs2232079(T) (TFBS variant, OR 0.88, 95%CI 0.83,0.93, p = 6.4 x 10-6) and rs10025845(A) (TFBS variant, OR 1.88, 95%CI 1.50,1.12, p = 1.32 x 10-5). The SNP with the most significant association, rs2472632, is located in an enhancer predicted to target the coiled-coil domain containing 34 oncogene. Our results provide new insights into genetic risk factors for PDAC by a focused analysis of polymorphisms in regulatory regions and demonstrating the usefulness of functional prioritization to identify loci associated with PDAC risk.
Mesencephalic Locomotor Region and Presynaptic Inhibition during Anticipatory Postural Adjustments in People with Parkinson's Disease
(2024) SILVA-BATISTA, Carla; LIRA, Jumes; COELHO, Daniel Boari; LIMA-PARDINI, Andrea Cristina de; NUCCI, Mariana Penteado; MATTOS, Eugenia Casella Tavares; MAGALHAES, Fernando Henrique; BARBOSA, Egberto Reis; TEIXEIRA, Luis Augusto; AMARO JUNIOR, Edson; UGRINOWITSCH, Carlos; HORAK, Fay B.
Individuals with Parkinson's disease (PD) and freezing of gait (FOG) have a loss of presynaptic inhibition (PSI) during anticipatory postural adjustments (APAs) for step initiation. The mesencephalic locomotor region (MLR) has connections to the reticulospinal tract that mediates inhibitory interneurons responsible for modulating PSI and APAs. Here, we hypothesized that MLR activity during step initiation would explain the loss of PSI during APAs for step initiation in FOG (freezers). Freezers (n = 34) were assessed in the ON-medication state. We assessed the beta of blood oxygenation level-dependent signal change of areas known to initiate and pace gait (e.g., MLR) during a functional magnetic resonance imaging protocol of an APA task. In addition, we assessed the PSI of the soleus muscle during APA for step initiation, and clinical (e.g., disease duration) and behavioral (e.g., FOG severity and APA amplitude for step initiation) variables. A linear multiple regression model showed that MLR activity (R2 = 0.32, p = 0.0006) and APA amplitude (R2 = 0.13, p = 0.0097) explained together 45% of the loss of PSI during step initiation in freezers. Decreased MLR activity during a simulated APA task is related to a higher loss of PSI during APA for step initiation. Deficits in central and spinal inhibitions during APA may be related to FOG pathophysiology.
Maxillary sinus volumetric changes in jet aircraft pilots: A multislice computed tomography pilot study
(2023) SILVA, Yeda da; MUNHOZ, Luciana; PARGA FILHO, Jose Rodrigues; DAMASCENO, Andreza Gomes; ROSA, Cesar Felipe Franca da; ZUKOVSKI, Eduardo Bilaqui; TENG, Erik Zhu; CASTRO, Claudio Campi de
Purpose: This study evaluated maxillary sinus volume changes in military jet aircraft pilot candidates before and after the training program, in comparison with a control group, considering the effects of pressurization, altitude, and total flight hours, through multislice computed tomography.Materials and Methods: Fifteen fighter pilots were evaluated before initiating the training program and after the final approval. The control group consisted of 41 young adults who had not flown during their military career. The volumes of each maxillary sinus were measured individually before and at the end of the training program.Results: When comparing the initial and final volumes in the pilots, a statistically significant increase was observed both in the left and right maxillary sinuses. When evaluating the average total volume of the maxillary sinuses (i.e., the average volume of the right and left maxillary sinuses together), a significant increase in the volume of the maxillary sinuses was observed in the pilot group when compared to the control group.Conclusion: The maxillary sinus volumes in aircraft pilot candidates increased after the 8-month training program. This may be explained by changes in the gravitational force, the expansion of gas, and positive pressure from oxygen masks. This unprecedented investigation among pilots might lead to other investigations considering paranasal sinus alterations in this singular population. (Imaging Sci Dent 20220173)
A scan of all coding region variants of the human genome, identifies 13q12.2-rs9579139 and 15q24.1-rs2277598 as novel risk loci for pancreatic ductal adenocarcinoma
(2023) GIACCHERINI, Matteo; GORI, Leonardo; GENTILUOMO, Manuel; FARINELLA, Riccardo; CERVENA, Klara; SKIECEVICIENE, Jurgita; DIJK, Frederike; CAPURSO, Gabriele; VEZAKIS, Antonis; ARCHIBUGI, Livia; CHAMMAS, Roger; HUSSEIN, Tamas; TAVANO, Francesca; HEGYI, Peter; LOVECEK, Martin; IZBICKI, Jakob R.; BRENNER, Hermann; MOHELNIKOVA-DUCHONOVA, Beatrice; DELL'ANNA, Giuseppe; KUPCINSKAS, Juozas; ERMINI, Stefano; AOKI, Mateus Nobrega; NEOPTOLEMOS, John P.; GAZOULI, Maria; PASQUALI, Claudio; PEZZILLI, Raffaele; TALAR-WOJNAROWSKA, Renata; OLIVERIUS, Martin; AL-SAEEDI, Mohammed; LUCCHESI, Maurizio; FURBETTA, Niccolo; CARRARA, Silvia; EIJCK, Casper H. J. van; MALECKAS, Almantas; MILANETTO, Anna Caterina; LAWLOR, Rita T.; SCHOETTKER, Ben; BOGGI, Ugo; MORELLI, Luca; GINOCCHI, Laura; PISANI, Ruggero Ponz de Leon; SPERTI, Cosimo; ZERBI, Alessandro; ARCIDIACONO, Paolo Giorgio; UZUNOGLU, Faik G.; BUNDUC, Stefania; HOLLECZEK, Bernd; GIOFFREDA, Domenica; MALECKA-WOJCIESKO, Ewa; KIUDELIS, Mindaugas; SZENTESI, Andrea; LAARHOVEN, Hanneke W. M. van; SOUCEK, Pavel; GOETZ, Mara; EROSS, Balint; CAVESTRO, Giulia Martina; BASSO, Daniela; PERRI, Francesco; LANDI, Stefano; CANZIAN, Federico; CAMPA, Daniele
Coding sequence variants comprise a small fraction of the germline genetic variability of the human genome. However, they often cause deleterious change in protein function and are therefore associated with pathogenic phenotypes. To identify novel pancreatic ductal adenocarcinoma (PDAC) risk loci, we carried out a complete scan of all common missense and synonymous SNPs and analysed them in a case-control study comprising four different populations, for a total of 14 538 PDAC cases and 190 657 controls. We observed a statistically significant association between 13q12.2-rs9581957-T and PDAC risk (P = 2.46 x 10(-9)), that is in linkage disequilibrium (LD) with a deleterious missense variant (rs9579139) of the URAD gene. Recent findings suggest that this gene is active in peroxisomes. Considering that peroxisomes have a key role as molecular scavengers, especially in eliminating reactive oxygen species, a malfunctioning URAD protein might expose the cell to a higher load of potentially DNA damaging molecules and therefore increase PDAC risk. The association was observed in individuals of European and Asian ethnicity. We also observed the association of the missense variant 15q24.1-rs2277598-T, that belongs to BBS4 gene, with increased PDAC risk (P = 1.53 x 10(-6)). rs2277598 is associated with body mass index and is in LD with diabetes susceptibility loci. In conclusion, we identified two missense variants associated with the risk of developing PDAC independently from the ethnicity highlighting the importance of conducting re-analysis of genome-wide association studies (GWASs) in light of functional data. [GRAPHICS] .
Patentometric analysis as a subsid to the management of technological innovation: a case study in radiopharmaceuticals for cancer
(2023) CHAVES, Henrique Koch; SILVA, Julia Maria Brandao da; SILVEIRA, Carla Cristina de Freitas da; MARQUES, Fabio Luiz Navarro; MAGALHAES, Jorge Lima de
Objective: Prospect the evolution of patent applications onIdentify, extract, process data, organize and make available technological information in the form of graphical interfaces described in oncology patents containing the radioisotopes gallium-68 (Ga-68), copper-64 (Cu-64) and zirconium-89 ((89)Z) in order to understand the development of science and technology in the area. Method: The ORBIT Intelligence (R) platform from the company QUESTEL (R) was used to identify, extract, process the data, organize and make available the technological information in the form of graphical interfaces, analysis of the filed patents in force in the last 20 years in the main patent offices in the world. Result: For gallium-68, copper-64 and zirconium-89, respectively, 36, 18 and 10 families of patents containing the search terms described in the methodology were identified. In both cases, China stands out in the number of filed patent applications, depositing 61% of the total analyzed. It was also observed that many of the analyzed documents were deposited by universities, reinforcing the concept of company-university partnership. The results obtained point out that the area of this study continues to grow and the understanding of the information present in the patent documents makes it possible to reflect on spaces to intensify the development of innovations in the oncology area. Conclusions: The results obtained demonstrate the relevance of information for the management of a country's technological development, which can serve as an efficient method to analyze, compare and monitor research and development activities in cancer and also support the planning processes organizations, as well as supporting public and private health policies.
Assessment of bioactive peptides derived from laminin-111 as prospective breast cancer-targeting agents
(2024) MENDONCA, Fernanda Ferreira; SOBRAL, Danielle Vieira; DURANTE, Ana Claudia Ranucci; MIRANDA, Ana Claudia Camargo; MEJIA, Jorge; FARIA, Daniele de Paula; MARQUES, Fabio Luiz Navarro; BARBOZA, Marycel Figols de; FUSCALDI, Leonardo Lima; MALAVOLTA, Luciana
Breast cancer remains a pressing public health issue primarily affecting women. Recent research has spotlighted bioactive peptides derived from laminin-111, implicated in breast tumor development. Remarkably, the sequences IKVAV, YIGSR, and KAFDITYVRLKF from the alpha 1, beta 1, and gamma 1 chains, respectively, have garnered significant attention. This study aims to assess the potential of these radiolabeled peptides as targeting agents for breast cancer. The three peptides were synthesized using the Fmoc strategy, purified via reversed-phase high-performance liquid chromatography (RP-HPLC), and characterized through mass spectrometry. Iodine-131 (131I) radiolabeling was performed using the chloramine T method, exhibiting high radiochemical yield and stability for [131I]I-YIKVAV and [131I]I-YIGSR. Conversely, [131I]I-KAFDITYVRLKF demonstrated low radiochemical yield and stability and was excluded from the biological studies. The lipophilicity of the compounds ranged from - 2.12 to - 1.10. Serum protein binding assay for [131I]I-YIKVAV and [131I]I-YIGSR reached approximately equal to 48% and approximately equal to 25%, respectively. Affinity for breast cancer cells was evaluated using MDA-MB-231 and MCF-7 tumor cell lines, indicating the affinity of the radiopeptides with these tumor cells. Ex vivo biodistribution profiles of the radiopeptides were assessed in the MDA-MB-231 breast tumor animal model, revealing tumor tissue accumulation, supported by a high tumor-to-contralateral muscle ratio and autoradiography. These results signify the effective penetration of YIKVAV and YIGSR into tumor tissue. Therefore, the synthesized alpha 1 and beta 1 peptide fragments exhibit favorable characteristics as potential breast cancer-targeting agents, promising future exploration as radiopharmaceuticals for breast cancer.