LIM/59 - Laboratório de Biologia Celular

URI Permanente desta comunidade

https://observatorio.fm.usp.br/handle/OPI/3830
O Laboratório de Biologia Celular é ligado ao Departamento de Patologia da Faculdade de Medicina da Universidade de São Paulo (FMUSP).

Linhas de pesquisa: sistema reprodutor: estudo experimental das alterações fisiopatológicas da função reprodutiva; sistema respiratório: estudo do remodelamento pulmonar e dos mecanismos de defesa pulmonar; sistema cardiovascular: estudo experimental dos mecanismos controle da pressão arterial. Temas gerais: biopatologia da matriz extracelular; aplicação de métodos morfométricos ao estudo do remodelamento tecidual; ultraestrutura e função celular.

Site oficial: http://limhc.fm.usp.br/portal/lim59-laboratorio-de-biologia-celular/

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Treatment with empagliflozin improves cardiac function in infarcted animals associated with increased baroreflex sensitivity
(2023) SILVA, B. Da; NASCIMENTO-CARVALHO, B.; SOUZA, L. I. De; SILVA, M. B. Da; MARQUES, J. R.; DOURADO, P. M. M.; CONSOLIM-COLOMBO, F.; IRIGOYEN, M. C. C.
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Aerobic Exercise Effects in Severe Asthma: Involvement of Lung Fibroblast (MRC-5) Cells and Kinins Signaling
(2021) BRANDAO-RANGEL, Maysa A. Rodrigues; MORAES-FERREIRA, Renilson; SILVA-REIS, Anamei; PALMEIRA, H. S. Palmeira; ALMEIDA, Francine M.; OLIMPIO, R. S. Olimpio; ZAMARIOLI, Lucas S.; OLIVEIRA, Carlos R.; DAMACENO-RODRIGUES, Nilsa R.; PESQUERO, Joao B.; AIMBIRE, Flavio; VIEIRA, Rodolfo De Paula
article 1 Citação(ões) na Scopus
Strongyloides infection screening in transplant candidates: What is the best strategy?
(2023) GRYSCHEK, Ronaldo Cesar Borges; CORRAL, Marcelo Andreetta; SITTA, Renata Barnabe; GOTTARDI, Maiara; PIERROTTI, Ligia Camera; COSTA, Silvia Figueiredo; ABDALA, Edson; CHIEFFI, Pedro Paulo; PAULA, Fabiana Martins de
Background: The potential that Strongyloides stercoralis infection has to cause major morbidity and high mortality when the disseminated form occurs in transplant patients is of particular concern.Methods: In this study, the objective was to observe S. stercoralis infection in patients who are candidates for transplantation by using parasitological, serological, and molecular techniques and to propose an algorithm for the detection of that infection in transplant candidates.Results: By parasitological techniques, 10% of fecal samples were positive. Anti-Strongyloides antibodies immunoglobulin G were detected in 19.3% and 20.7% of patients by immunofluorescence assay and enzyme-linked immunosorbent assay, respectively. S. stercoralis DNA was observed in 17.3% of samples by conventional polymerase chain reaction and 32.7% of samples by quantitative polymerase chain reaction (qPCR).Conclusion: The set of results allows us to reinforce that a positive result by parasitological techniques and/or qPCR indicates that the specific treatment should be applied. However, the improvement of diagnostic techniques may suggest changes in the screening for strongyloidiasis in these patients. image
article 3 Citação(ões) na Scopus
Neurovascular and hemodynamic responses to mental stress and exercise in severe COVID-19 survivors
(2023) FARIA, Diego; MOLL-BERNARDES, Renata; TESTA, Laura; MONIZ, Camila M. V.; RODRIGUES, Erika C.; MOTA, Jose M.; SOUZA, Francis R.; ALVES, Maria Janieire N. N.; ONO, Bruna E.; IZAIAS, Joao E.; SALES, Artur O.; RODRIGUES, Thais S.; SALEMI, Vera M. C.; JORDAO, Camila P.; ANGELIS, Katia De; CRAIGHEAD, Daniel H.; ROSSMAN, Matthew J.; BORTOLOTTO, Luiz A.; CONSOLIM-COLOMBO, Fernanda M.; IRIGOYEN, Maria C. C.; SEALS, Douglas R.; NEGRAO, Carlos E.; SALES, Allan R. K.
Previous studies show that COVID-19 survivors have elevated muscle sympathetic nerve activity (MSNA), endothelial dysfunction, and aortic stiffening. However, the neurovascular responses to mental stress and exercise are still unexplored. We hypothesized that COVID-19 survivors, compared with age-and body mass index (BMI)-matched control subjects, exhibit abnormal neurovascular responses to mental stress and physical exercise. Fifteen severe COVID-19 survivors (aged: 49 +/- 2 yr, BMI: 30 +/- 1 kg/m(2)) and 15 well-matched control subjects (aged: 46 +/- 3 yr, BMI: 29 +/- 1 kg/m(2)) were studied. MSNA (microneurography), forearm blood flow (FBF), and forearm vascular conductance (FVC, venous occlusion plethysmography), mean arterial pressure (MAP, Finometer), and heart rate (HR, ECG) were measured during a 3-min mental stress (Stroop Color-Word Test) and during a 3-min isometric handgrip exercise (30% of maximal voluntary contraction). During mental stress, MSNA (frequency and incidence) responses were higher in COVID-19 survivors than in controls (P < 0.001), and FBF and FVC responses were attenuated (P < 0.05). MAP was similar between the groups (P > 0.05). In contrast, the MSNA (frequency and incidence) and FBF and FVC responses to handgrip exercise were similar between the groups (P > 0.05). MAP was lower in COVID-19 survivors (P < 0.05). COVID-19 survivors exhibit an exaggerated MSNA and blunted vasodilatory response to mental challenge compared with healthy adults. However, the neurovascular response to handgrip exercise is preserved in COVID-19 survivors. Overall, the abnormal neurovascular control in response to mental stress suggests that COVID-19 survivors may have an increased risk to cardiovascular events during mental challenge.
article 0 Citação(ões) na Scopus
Effect of aerobic and resistance exercise training on endothelial function in individuals with overweight and obesity: a systematic review with meta-analysis of randomized clinical trials
(2023) CORTES, Maiquel Bueno; SILVA, Raphael Silveira Nunes da; OLIVEIRA, Patricia Caetano de; SILVA, Diego Silveira da; IRIGOYEN, Maria Claudia Costa; WACLAWOVSKY, Gustavo; SCHAUN, Maximiliano Isoppo
The objective of this systematic review was to examine the effects of exercise training on endothelial function in individuals with overweight and obesity. Our review study included only randomized controlled trials (RCTs) involving adults (>= 18 years of age) with body mass index (BMI)>= 25.0 kg/m(2). Our search was conducted in the electronic bases MEDLINE (PubMed), Cochrane, LILACS and EMBASE and in the gray literature. We performed random-effects analyses for effect estimates and used 95% prediction intervals (95% PI) for estimating the uncertainty of the study results. There were selected 10 RCTs involving 14 groups (n=400). The quality assessment of studies using Cochrane risk-of-bias 2 (RoB 2) tool identified some concerns. Exercise training resulted in improved flow-mediated dilation (FMD) in individuals with overweight and obesity (p<0.001) compared to the no-exercise control group. This effect of training modalities on FMD was seen for aerobic training (p<0.001) but not for resistance training (p=0.051). There was no difference in FMD in response to exercise training by BMI classification (overweight, obesity, overweight+obesity), p=0.793. The present results are consistent with the notion that aerobic exercise training elicits favorable adaptations in endothelial function in individuals with overweight and obesity. Our findings should be interpreted with caution because of the small number of studies included in this review.
article 1 Citação(ões) na Scopus
Anserine is expressed in human cardiac and skeletal muscles
(2023) GONCALVES, Livia de Souza; PEREIRA, Wagner Ribeiro; SILVA, Rafael Pires da; YAMAGUCHI, Guilherme Carvalho; CARVALHO, Victor Henrique; VARGAS, Bianca Scigliano; JENSEN, Leonardo; MEDEIROS, Marisa Helena Gennari de; ROSCHEL, Hamilton; ARTIOLI, Guilherme Giannini
We evaluated whether anserine, a methylated analog of the dipeptide carnosine, is present in the cardiac and skeletal muscles of humans and whether the CARNMT1 gene, which encodes the anserine synthesizing enzyme carnosine-N-methyltransferase, is expressed in human skeletal muscle. We found that anserine is present at low concentrations (low micromolar range) in both cardiac and skeletal muscles, and that anserine content in skeletal muscle is similar to 15 times higher than in cardiac muscle (cardiac muscle: 10.1 +/- 13.4 mu mol.kg(-1) of dry muscle, n = 12; skeletal muscle: 158.1 +/- 68.5 mu mol.kg(-1) of dry muscle, n = 11, p < 0.0001). Anserine content in the heart was highly variable between individuals, ranging from 1.4 to 45.4 mu mol.kg(-1) of dry muscle, but anserine content was not associated with sex, age, or body mass. We also showed that CARNMT1 gene is poorly expressed in skeletal muscle (n = 10). This is the first study to demonstrate that anserine is present in the ventricle of the human heart. The presence of anserine in human heart and the confirmation of its expression in human skeletal muscle open new avenues of investigation on the specific and differential physiological functions of histidine dipeptides in striated muscles.
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Perivascular Innervation in the Nasal Mucosa and Clinical Findings in Patients with Allergic Rhinitis and Idiopathic Rhinitis
(2023) CARVALHO, Thiago; JR, Joao Ferreira de Mello; CALDINI, Elia Tamaso Espin Garcia; SALGADO, Daniel Calduro; CARVALHO, Nicole Mary Garcia de; DAMACENO-RODRIGUES, Nilsa Regina; VOEGELS, Richard Louis
Introduction The nonspecific hyperreactivity of rhinitis has been attributed to neurotrophins activating sensory nerves and inflammatory cells. The relationship between these markers and the intensity of the symptoms is not well established and few studies have evaluated individuals with idiopathic rhinitis.Objective The present study aims to evaluate whether perivascular innervation and nerve growth factor (NGF) are related to the intensity of the clinical conditions in allergic rhinitis (AR) and idiopathic rhinitis (IR).Methods A total of 15 patients with AR and 15 patients with IR with the indication for inferior turbinectomy (associated or not with septoplasty) were selected. The patients received a score according to their signs and symptoms. After the surgery, we quantified eosinophils, mast cells, NGF, and nerve fibers in the nasal turbinate.Results The score of the signs and symptoms was higher in the AR group. Nerve growth factor was found in the cytoplasm of inflammatory cells in the submucosa in greater quantity in the AR group. The nerve fibers were distributed throughout the tissue, mainly in the subepithelial, glandular, and vascular regions, and there was no difference between the groups. Greater perivascular innervation was associated with a higher signs and symptoms score.Conclusions We concluded that these findings suggest that the NGF produced by submucosal inflammatory cells stimulates increased perivascular innervation in rhinitis, thus directly reflecting in more intense clinical conditions, especially in AR.
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EXERCISE TRAINING ASSOCIATED WITH DIETARY ADJUSTMENT INDUCES METABOLIC AND CARDIOVASCULAR BENEFITS IN HIGH-FAT-FED MENOPAUSE RATS
(2019) SANTOS, Adriano dos; SANTOS, Nicolas da Costa; NASCIMENTO-CARVALHO, Bruno do; CARVALHO, Sabrina Leonardo; MELLO-SILVA, Fernanda Queiroz; STABILE, Gabriel Correia; IZAIAS, Joao Eduardo; IRIGOYEN, Maria-Claudia; ANGELIS, Katia De; SCAPINI, Katia Bilhar; SANCHES, Iris Callado
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DIFFERENT VOLUMES OF EXERCISE TRAINING IN AN EXPERIMENTAL MODEL OF MENOPAUSE AND OBESITY: METABOLIC, HEMODYNAMIC AND AUTONOMIC EFFECTS
(2020) SANTOS, Adriano dos; COSTA-SANTOS, Nicolas da; BATISTA, Thayna Ribeiro; JESUS, Ney de; IZAIAS, Joao; NASCIMENTO-CARVALHO, Bruno do; ANGELIS, Katia De; IRIGOYEN, Maria-Claudia Costa; CAPERUTO, Erico; SCAPINI, Katia; SANCHES, Iris
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CARDIORENAL DYSFUNCTION IN MICE SUBMITTED TO AORTIC STENOSIS AND TREATED WITH SODIUM OXALATE
(2023) SILVA, Amanda; MARQUES, Juliana; NASCIMENTO, Bruno; SOUZA, Leandro; SILVA, Maikon; BENETTI, Acaris; IRIGOYEN, Maria Claudia