Core Promoter and Pre-Core Variants of the Hepatitis B Virus (HBV) Are Frequent in Chronic Hepatitis B HBeAg-Negative Patients Infected by Genotypes A and D

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Citações na Scopus
0
Tipo de produção
article
Data de publicação
2023
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
MDPI
Autores
DUQUE, Ulisses Horst
PERINI, Waltesia
LEWIS-XIMENEZ, Lia L.
VILLAR, Livia Melo
ESPUL, Carlos Alberto
Citação
VIRUSES-BASEL, v.15, n.12, article ID 2339, 11p, 2023
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
In Brazil, hepatitis B virus endemicity is low, moderate, or high in some areas, such as Espirito Santo State in the southeast region. In this study, we intend to characterize the basal core promoter (BCP) and pre-core region (PC) variants and their association with clinical/epidemiological disease patterns in patients infected with genotypes A and D. The study included 116 chronic hepatitis B patients from Espirito Santo State, Southeast Brazil, infected with genotypes A and D. Basal core promoter (BCP) and pre-core mutations were analyzed in these patients. The frequency of BCP and PC mutations was compared with age, HBeAg status, HBV genotype and subgenotype, HBV-DNA level, clinical classification, and transmission route. HBeAg-negative status was found in 101 (87.1%) patients: 87 (75.0%) were infected with genotype A (A1 = 85; A2 = 2) and 29 (25.0%) were infected with genotype D (D3 = 24; D4 = 3; D2 = 2). BCP + PC variants altogether were more frequent (48.1%) in genotype D than in genotype A strains (6.0%) (p < 0.001). When this evaluation was performed considering the cases that presented only the A1762T and/or G1764A (BCP) mutations, it was observed that the frequency was higher in genotype A (67.5%) compared to genotype D (7.4%) (p < 0.001). On the other hand, considering the samples with mutations only in positions G1896A and/or G1899A (PC), the frequency was higher in genotype D (75.8%) than in genotype A (6.9%) (p < 0.001). Interestingly, HBV DNA was lower than 2000 IU/mL especially when both BCP/PC mutations were present (p < 0.001) or when only PC mutations were detected (p = 0.047), reinforcing their role in viral replication.
Palavras-chave
chronic hepatitis B, HBV, pre core mutations, basal core promoter mutations, BCP and PC variants
URI
https://observatorio.fm.usp.br/handle/OPI/58038
Referências
  1. Agarwal A K, 2015, Med J Armed Forces India, V71, P225, DOI 10.1016/j.mjafi.2015.04.003
  2. Alexopoulou A, 2014, WORLD J GASTROENTERO, V20, P7644, DOI 10.3748/wjg.v20.i24.7644
  3. Bertolini DA, 2012, INFECT GENET EVOL, V12, P1295, DOI 10.1016/j.meegid.2012.04.009
  4. Chachá SGF, 2017, MEM I OSWALDO CRUZ, V112, P626, DOI 10.1590/0074-02760160540
  5. Ghosh S, 2012, CLIN MICROBIOL INFEC, V18, pE412, DOI 10.1111/j.1469-0691.2012.03975.x
  6. Gomes-Gouvêa MS, 2015, ANTIVIR THER, V20, P387, DOI 10.3851/IMP2938
  7. Günther S, 1998, J GEN VIROL, V79, P375
  8. Jammeh S, 2008, J GEN VIROL, V89, P901, DOI 10.1099/vir.0.83468-0
  9. Kramvis A, 2013, ANTIVIR THER, V18, P513, DOI 10.3851/IMP2657
  10. Lago BV, 2019, VIRUSES-BASEL, V11, DOI 10.3390/v11090860
  11. Lampe E, 2017, J GEN VIROL, V98, P1389, DOI 10.1099/jgv.0.000789
  12. Lau DTY, 2021, HEPATOLOGY, V73, P1637, DOI 10.1002/hep.31506
  13. Lee MH, 2012, INTERVIROLOGY, V55, P296, DOI 10.1159/000329941
  14. Leng XH, 2015, MOL MED REP, V12, P5141, DOI 10.3892/mmr.2015.4072
  15. Lin CL, 2008, J BIOMED SCI, V15, P137, DOI 10.1007/s11373-007-9225-8
  16. de Mello FMMA, 2014, REV SOC BRAS MED TRO, V47, P701, DOI 10.1590/0037-8682-0158-2014
  17. Nordin M, 2014, J MED VIROL, V86, P437, DOI 10.1002/jmv.23839
  18. Nowak MA, 1996, P NATL ACAD SCI USA, V93, P4398, DOI 10.1073/pnas.93.9.4398
  19. O'Brien M, 2015, CAN J GASTROENTEROL, V29, pE1, DOI 10.1155/2015/940825
  20. Reuter TQ, 2022, ANN HEPATOL, V27, DOI 10.1016/j.aohep.2021.100574
  21. Rezende REF, 2005, J CLIN VIROL, V32, P53, DOI 10.1016/j.jcv.2004.08.001
  22. Siegel S., 2006, Estatistica Nao-Parametrica Para Ciencias Do Comportamento, V32nd
  23. Sitnik R, 2004, J CLIN MICROBIOL, V42, P2455, DOI 10.1128/JCM.42.6.2455-2460.2004
  24. Eloy AMX, 2013, BRAZ J INFECT DIS, V17, P704, DOI 10.1016/j.bjid.2013.03.011

Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/01
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/07
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