SHEILA DE OLIVEIRA GARCIA MATEOS
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- Parvovirus among Patients with Cytopenia of Unknown Origin in Brazil: a Case-Control Study(2011) GARCIA, Sheila de Oliveira; KLEINE NETO, Walter; COSTA, Antonio Charlys da; SANABANI, Sabri Saeed; MENDRONE JR., Alfredo; PEREIRA, Juliana; SABINO, Ester CerdeiraThe molecular prevalence of human parvovirus B19V (B19V) in bone marrow (BM) samples from 120 cases with cytopenias of unknown etiology was compared with that in samples from 45 BM donors (control group 1) and 120 oncohematological patients (control group 2) to determine the role that B19V genotypes may play in unexplained cytopenias. Of the 285 participants, the BM samples of 39 (13.7%) contained B19V DNA (21 with genotype 1, 5 with genotype 2, and 13 with genotype 3). The prevalences of B19V were similar between case and control subjects (15.0% versus 12.7%, respectively). Genotypes 2 and 3 were associated with older age and were detected in similar proportions between case and control group 2 subjects. The results of this study do not support a role for B19V genotype variants in the etiology of unexplained cytopenias.
- 10-year analysis of human immunodeficiency virus incidence in first-time and repeat donors in Brazil(2021) MATEOS, Sheila de Oliveira Garcia; PREISS, Liliana; GONCALEZ, Thelma T.; OLIVEIRA, Claudia Di Lorenzo; GREBE, Eduard; GERMANIO, Clara Di; STONE, Mars; AMORIM FILHO, Luiz; PROIETTI, Anna Barbara Carneiro; BELISARIO, Andre Rolim; ALMEIDA-NETO, Cesar de; MENDRONE-JUNIOR, Alfredo; LOUREIRO, Paula; BUSCH, Michael P.; CUSTER, Brian; SABINO, Ester CerdeiraBackground and objectives Incidence in first-time and repeat blood donors is an important measure of transfusion-transmitted HIV infection (TT-HIV) risk. This study assessed HIV incidence over time at four large blood centres in Brazil. Materials and methods Donations were screened and confirmed using serological assays for HIV from 2007 to 2016, and additionally screened by nucleic acid testing from 2011 forward. Limiting antigen (LAg) avidity testing was conducted on HIV seroreactive samples from first-time donors to classify whether an infection was recently acquired. We calculated incidence in first-time donors using the mean duration of recent infection and in repeat donors using classical methods. Time and demographic trends were assessed using Poisson regression. Results Over the 10-year period, HIV incidence in first-time donors was highest in Recife (45 center dot 1/100 000 person-years (10(5)py)) followed by Sao Paulo (32 center dot 2/10(5)py) and then Belo Horizonte (23 center dot 3/10(5)py), and in repeat donors was highest in Recife (33 center dot 2/10(5)py), Belo Horizonte (27 center dot 5/10(5)py) and Sao Paulo (17 center dot 0/10(5)py). Results from Rio de Janeiro were available from 2013 to 2016 with incidence in first-time donors of 35 center dot 9/10(5)py and repeat donors from 2011 to 2016 of 29 center dot 2/10(5)py. Incidence varied by other donor demographics. When incidence was considered in 2-year intervals, no significant trend was evident. Overall residual risk of TT-HIV was 5 center dot 46 and 7 center dot 41 per million units of pRBC and FFP transfused, respectively. Conclusion HIV incidence in both first-time and repeat donors varied by region in Brazil. Clear secular trends were not evident.
- Seroprevalence of anti-SARS-CoV-2 among blood donors in Rio de Janeiro, Brazil(2020) AMORIM FILHO, Luiz; SZWARCWALD, Celia Landmann; MATEOS, Sheila de Oliveira Garcia; LEON, Antonio Carlos Monteiro Ponce de; MEDRONHO, Roberto de Andrade; VELOSO, Valdilea Goncalves; LOPES, Josiane Iole Franca; PORTO, Luis Cristovao de Moraes Sobrino; CHIEPPE, Alexandre; WERNECK, Guilherme LoureiroOBJECTIVE: To estimate the seroprevalence of antibodies to SARS-CoV-2 among blood donors in the state of Rio de Janeiro, Brazil. METHODS: Data were collected on 2,857 blood donors from April 14 to 27, 2020. This study reports crude prevalence of antibodies to SARS-CoV-2, population weighted prevalence for the state, and prevalence adjusted for test sensitivity and specificity. Logistic regression models were used to establish the correlates of SARS-CoV-2 prevalence. For the analysis, we considered collection period and site, sociodemographic characteristics, and place of residence. RESULTS: The proportion of positive tests for SARS-Cov-2, without any adjustment, was 4.0% (95%CI 3.3-4.7%), and the weighted prevalence was 3.8% (95%CI 3.1-4.5%). We found lower estimates after adjusting for test sensitivity and specificity: 3.6% (95%CI 2.7-4.4%) for the non-weighted prevalence, and 3.3% (95%CI 2.6-4.1%) for the weighted prevalence. Collection period was the variable most significantly associated with crude prevalence: the later the period, the higher the prevalence. Regarding sociodemographic characteristics, the younger the blood donor, the higher the prevalence, and the lower the education level, the higher the odds of testing positive for SARS-Cov-2 antibody. We found similar results for weighted prevalence. CONCLUSIONS: Our findings comply with some basic premises: the increasing trend over time, as the epidemic curve in the state is still on the rise; and the higher prevalence among both the youngest, for moving around more than older age groups, and the less educated, for encountering more difficulties in following social distancing recommendations. Despite the study limitations, we may infer that Rio de Janeiro is far from reaching the required levels of herd immunity against SARS-CoV-2.
bookPart O vírus e suas variantes(2021) MATEOS, Sheila de Oliveira Garcia; SABINO, Ester Cerdeira- Comparison of Two Methods of Capillary Sampling in Blood Pre-Donation Anemia Screening in Brazil(2023) FLOR, Cristina Rabelo; BALDONI, Andre de Oliveira; MATEOS, Sheila de Oliveira Garcia; SABINO, Ester Cerdeira; OLIVEIRA, Claudia Di LorenzoBackground: The laboratory tests most used by blood banks to diagnose anemia are the hemoglobin (Hb) and microhematocrit (Hct) tests, measured from capillary samples. Objective: To analyze the two capillary screening methods for pre-donation anemia by comparing their agreement in diagnosing anemia. Method: A cross-sectional study in a population of 15,521 blood donation candidates for whom information was available on Hb and Hct, performed from capillary blood samples. Hb was determined using the HemoCue(& REG;) test and Hct by the centrifugation method. The Kappa coefficient was calculated to assess the agreement between the methods. Pearson's correlation tests and gender-adjusted linear regression were used to assess the change in the response variable (Hb) as a function of the explanatory variable (Hct). Results: The majority of the study population were men (70.4%), aged between 18 and 44 years (72.1%), who declared themselves white or mixed skin color (85.6%), and had undergone at least 11 years of complete education (72.4%). The Kappa coefficient found was 92.7 and 99.2 for women and men, respectively. Pearson's correlation showed a correlation coefficient of 0.98 and the linear regression graph showed an adequate relationship between the tests with R-2 = 0.97. Conclusions: Comparing the Hb and Hct capillary tests, it was found that Hct can be safely used to screen for anemia in pre-blood donation.
- Association of HIV infection with clinical and laboratory characteristics of sickle cell disease(2020) BELISARIO, Andre Rolim; BLATYTA, Paula F.; VIVANCO, Diana; OLIVEIRA, Claudia Di Lorenzo; CARNEIRO-PROIETTI, Anna Barbara; SABINO, Ester Cerdeira; ALMEIDA-NETO, Cesar de; LOUREIRO, Paula; MAXIMO, Claudia; MATEOS, Sheila de Oliveira Garcia; V, Miriam Flor-Park; RODRIGUES, Daniela de Oliveira Werneck; MOTA, Rosimere Afonso; GONCALEZ, Thelma T.; HOFFMANN, Thomas J.; KELLY, Shannon; CUSTER, BrianBackground Sickle cell disease (SCD) is a multisystem disorder characterized by a wide spectrum of clinical manifestations and severity. Studies investigating potential effects of co-morbid human immunodeficiency virus (HIV) and SCD have produced conflicting results, and additional investigations are needed to elucidate whether the interaction between the two disease states might impact both HIV and SCD clinical outcomes. The association of HIV infection with clinical and laboratory characteristics of patients with SCD was assessed. Methods This nested case-control study included individuals with SCD with HIV treated at six Brazilian SCD centers. Clinical and laboratory data were abstracted from medical records. HIV positive participants were compared to age, gender, center, and SCD genotype matched HIV negative participants (ratio 1:4). Individual clinical outcomes as well as a composite outcome of any SCD complication and a composite outcome of any HIV-related complication were compared between the two groups. Results Fifteen HIV positive participants were included, 12 (80%) alive and 3 (20%) deceased. Most of the HIV positive patients had HbSS (60%;n = 9), 53% (n = 8) were female, and mean age was 30 +/- 13 years. The frequency of individual SCD complications of acute chest syndrome/pneumonia, sepsis/bacteremia, pyelonephritis, ischemic stroke, hemorrhagic stroke, abnormal transcranial Doppler (TCD), and pulmonary hypertension was higher in HIV positive participants when compared to HIV negative, although analyzed individually none were statistically significant. HIV positive participants had significantly higher risk of any SCD complication and of a composite HIV-related complication compared to the HIV negative group (HR = 4.6; 95%CI 1.1-19.6;P = 0.04 and HR = 7.7; 95%CI 1.5-40.2;P = 0.02, respectively). There was a non-significant trend towards higher risk of any infections in participants with HIV positive (HR = 3.5; 95%CI 0.92-13.4;P = 0.07). Laboratory parameters levels were not significantly different in individuals with and without HIV. Conclusions In summary, our study in SCD patients shows that those with HIV have an increased risk of any SCD complication and HIV-related complications, as well as a suggestive but not significantly increased risk of infections.