RICHARD YUDI HIDA
Índice h a partir de 2011
7
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
17 resultados
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Agora exibindo 1 - 10 de 17
article 1 Citação(ões) na Scopus Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits(2015) REGGI, Jose Ricardo de Abreu; HIDA, Richard Yudi; HIDA, Milton Massato; NISHIWAKI-DANTAS, Maria Cristina; SUZUKI, HisashiPurpose: To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.- Tacrolimus Ointment for Refractory Posterior Blepharitis(2017) SAKASSEGAWA-NAVES, Fernando Eiji; RICCI, Helena Maria Moraes; MOSCOVICI, Bernardo Kaplan; MIYAMOTO, Daniela Akemi; CHIACCHIO, Brenda Biagio; HOLZCHUH, Ricardo; SANTO, Ruth Muyuki; HIDA, Richard YudiPurpose: This prospective, randomized, double-blind interventional case series was designed to evaluate the short-term efficacy of 0.03% tacrolimus ointment as a new therapeutic approach for refractory cases of posterior blepharitis.Methods: Forty eyes (20 patients) with posterior blepharitis refractory to previous treatment were randomized. Eighteen eyes (9 patients) were treated with 0.03% tacrolimus ointment and 20 eyes (10 patients) with placebo ointment twice daily. Patients were evaluated with a questionnaire and slit-lamp examination 14days and 28days after treatment, and symptoms and signs of blepharitis were compared to those observed at baseline.Results: We could observe statistical difference in the outcome measurements of meibomian gland secretion, conjunctival hyperemia, telangiectasia of inferior lid, Rose Bengal, and fluorescein scoring for the study group. As for the symptoms score, we observed statistical difference in the symptoms scoring for pruritus and dry eye sensation in the tacrolimus group.Conclusion: This study suggests that topical administration of 0.03% tacrolimus ointment can improve some symptoms and some ocular surface status in patients with refractory posterior blepharitis.
- Grooved and nongrooved clear corneal incisions in phacoemulsification: permeability study(2016) BERGAMASCO, Victor D.; ANTUNES, Victor A. C.; HIDA, Richard Y.; MALAVAZZI, Gustavo R.Purpose: To detect the inflow of trypan blue through grooved and nongrooved sutureless self-sealing clear corneal incisions at the end of phacoemulsification as compared to a control group. Methods: A prospective randomized masked trial considered 52 eyes randomized into 3 groups in which phacoemulsification was performed: group A, nongrooved incisions; group B, grooved incisions; and group C, controls. By the end of each surgery, trypan blue was instilled on the ocular surface in groups A and B and rinsed out after 2 minutes. A sample of the anterior chamber content was collected and analyzed by high-performance liquid chromatography to identify and quantify the trypan blue concentration. The presence of trypan blue was expressed as a specific single peak graphic image. The mean areas of these peaks were used to assess the groups using a nonparametric Mann-Whitney test. Results: There was a statistically significant difference between the nongrooved incisions group and the control group (p = 0.0448). No significant difference was observed between group B (grooved incision) and controls (p = 0.1800). Conclusions: Trypan blue was detected in the anterior chamber when nongrooved clear corneal incision was used. There was no trypan blue detection in the group with grooved clear corneal main incisions.
- Topographic outcomes after corneal collagen crosslinking in progressive keratoconus: 1-year follow-up(2017) TIVERON JR., Mauro C.; PENA, Camila Ribeiro Koch; HIDA, Richard Yudi; MOREIRA, Luciane Bugmann; BRANCO, Felipe Roberto Exterhotter; KARA-JUNIOR, NewtonPurpose: We aimed to report and analyze topographic and refractive outcomes following corneal collagen crosslinking (CXL) in patients with progressive keratoconus (KC). Methods: We performed a retrospective, analytical, and observational study of 100 eyes from 74 progressive KC patients who underwent CXL at the Eye Hospital of Parana. Keratometric values were analyzed preoperatively as well as 3 and 12 months postoperatively. Results: For a total of 100 eyes, 68 belonged to male patients. The mean age of our study population was 19.9 +/- 5.61 years. The average visual acuity and topographic parameters overall were stable after 1 year (p<0.05). After 3 months, steepest keratometry reading (K2) and maximum keratometry (Kmax) were significantly decreased (p<0.05). Regarding topographic astigmatism (dK), there was no significant difference between the 3-month and 12-month follow-ups. When we made comparisons between genders following CXL, there were no significant differences related to the changes in Kmax, K2, and spectacle-corrected distance visual acuity (SCDVA). Conclusions: CXL promoted stabilization or improvement of keratometric values and visual acuity. We found that keratoconus apex stability may be achieved 3 months after the procedure. There was no significant difference in keratometric and refractive values measured between male and female patients.
- Trends in corneal transplantation from 2001 to 2016 in Brazil(2018) ALMEIDA, Hirlana Gomes; HIDA, Richard Yudi; KARA-JUNIOR, NewtonA retrospective and analytical study was conducted by using data from the National Transplantation System on 184,575 corneal transplantations performed between January 2001 and December 2016 in order to analyze the trends in corneal transplantation from 2001 to 2016 in Brazil. The Cochran-Armitage test, analysis of variance, and Duncan's multiple comparisons were used to verify the existence of trends, compare the mean values between regions, and verify the mean differences, respectively. A significance level of 5% was used for all tests. The analysis showed that there was a 2.4-fold increase in the number of corneal transplantations (from 6,193 [35.2 per million people (pmp)] to 14,641 [71 pmp]; p<0.001), a 50.7% increase in the efficacy of meeting the population's demand for corneal transplantation (from 35.3% to 53.2%; p<0.001), an 11-fold increase in the number of corneal transplantation centers (from 32 to 356), and a 2.5-fold increase in the number of corneal transplantation teams (from 276 to 688) in Brazil during the period studied. The waiting list for corneal transplantation decreased by 45.4% (from 23,549 [123 pmp] to 12,865 [62.4 pmp]; p<0.001), and the corneal transplantation teams performed about 19 corneal transplantations per year. The best indices were observed in the southern, midwestern, and southeastern regions, and the worst indices were in the northern and northeastern regions. Brazil has been improving its capacity to perform corneal transplantation in the past 16 years, although this improvement varies across regions. However, the population's demand for corneal transplantation is yet to be satisfactorily met, primarily due to the low number of corneal donations.
- Face masks can reduce the frequency of face touching: an observational crossover study(2022) FREITAS, Ana Vega Carreiro de; MAGALHAES, Andressa Miranda; CARRICONDO, Pedro Carlos; SHINZATO, Amaryllis Avakian; HIDA, Richard YudiObjectives: The purpose of this study was to analyze face-touching patterns with and without a face mask. Methods: The behavior of face touching with and without a mask during an interview was assessed in 40 individuals. The frequency of touching in different areas of the face covered by the mask was compared with areas not covered by the face mask. Results: There was an increase in the number of individuals who touched the hair and the eye when they were not wearing the mask. There was an increase in the number of touches on the lips and hair when individuals were not wearing the face mask. When analyzing the area covered by the face mask, no difference was observed in the number of touches while using or not using masks. However, when the area not covered by a face mask was analyzed, a higher number of touches in individuals without masks was observed when compared with individuals wearing masks. Conclusion: Using a face mask can reduce or change the face-touching patterns in normal individuals, especially in areas not covered by the mask. Using face masks can possibly reduce the chances of being infected by autoinoculation. (c) 2022 The Author(s).
conferenceObject Corneal Endothelial Severity Scoring System for Fuchs's Endothelial Dystrophy: Sectorial Corneal Endothelial Deterioration Imaging(2018) HOLZCHUH, Ricardo; HIDA, Richard Y.; CHAMMAS, Sabrina Marques; CARVALHO, Fbio Ursulino Reis; TEIXEIRA, Ivan Corso; ABIB, Fernando Cesar- In vitro antimicrobial analysis of aqueous humor after topical application of moxifloxacin hydrochloride 0.5%(2015) MIRANDA, Aline P. de; SILVA, Cely B.; MIMICA, Lycia M. J.; MOSCOVICI, Bernardo K.; MALAVAZZI, Gustavo R.; HIDA, Richard Y.PURPOSE: To evaluate the in vitro antimicrobial activity of aqueous humor in patients who had preoperative topical application of moxifloxacin hydrochloride 0.5%. SETTING: Department of Ophthalmology, Santa Casa de Misericordia de Sao Paulo, Brazil. DESIGN: Comparative case series. METHODS: Twenty-nine eyes from 29 cataract surgery patients were included in this study. In the study group (n = 15 eyes), 3 topical applications of moxifloxacin hydrochloride 0.5% were administered preoperatively; in the control group (n = 14 eyes), no topical applications were administered. Aqueous humor samples were collected and stored in sterile microtubes at -80 degrees C until analysis. Antimicrobial analysis was performed using standard strains with standard sterile filter paper disks. Inhibition halos were measured in millimeters, and both bactericidal and bacteriostatic effects were analyzed. RESULTS: Inhibition halos were observed on most of the study group plates except those with Streptococcus pneumoniae: Escherichia coli (13.93 mm +/- 0.64 [SD]), Klebsiella pneumoniae (10.63 +/- 0.61 mm), Staphylococcus aureus (7.47 +/- 0.68 mm), and S epidermidis (4.20 +/- 3.33 mm) The differences between the mean inhibition halo diameters were statistically significant (P < .0001) in all samples. No bactericidal effect was observed against any of the microorganisms studied. CONCLUSIONS: After topical application of moxifloxacin 0.5%, aqueous humor showed bacteriostatic effect against E coli, K pneumoniae, S aureus, and S epidermidis. No bactericidal effect was observed against any of the microorganisms evaluated. No antimicrobial effect against S pneumoniae was observed.
- Short-chain cyanoacrylates and long-chain cyanoacrylates (Dermabond) have different antimicrobial effects(2021) HIDA, Richard Yudi; SILVA, Cely Barreto; ROMERO-KUSABARA, Ivana Lopes; MIMICA, Lycia Mara JenneObjective To compare the antimicrobial effect in vitro of a short-chain cyanoacrylate with a long-chain cyanoacrylate (Dermabond, Ethicon, Johnson and Johnson, USA) against bacterial strains. Methods and analysis The following bacterial strains were analysed: Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. For each microorganism, standardised sterile discs (6 mm) containing 10 mu L of ethyl-cyanoacrylate and 2-octyl cyanoacrylate were applied to the plate. All plates received a blank filter-paper disc with no adhesive (control). All plates were incubated for 24 hours, after which the bacterial inhibitory halos, if present, were measured in millimetres in its greater length. Results Inhibitory halos were observed for both adhesives for S. aureus. Inhibition halos were observed only for ethyl-cyanoacrylate for K. pneumoniae and E. coli. No inhibition halo was observed for P. aeruginosa in any sample. The relationship between the total size of the inhibition halos and the diameter of the paper filter for S. aureus was statistically significant compared with 2-octyl cyanoacrylate. Conclusion Data shown conclude that ethyl-cyanoacrylate showed in vitro bacteriostatic activity for S. aureus, E. coli and K. pneumoniae. 2-Octyl cyanoacrylate showed in vitro lower bacteriostatic activity only against S. aureus when compared with ethyl-cyanoacrylate. No in vitro bactericidal activity of ethyl-cyanoacrylate or 2-octyl cyanoacrylate was observed.
- Post-Photorefractive Keratectomy Contact Lens Microbiological Findings of Individuals Who Work in a Hospital Environment(2015) PEREIRA, Carlos Eduardo Goncalves; HIDA, Richard Yudi; SILVA, Cely Barreto; ANDRADE, Marizilda Rita de; FIORAVANTI-LUI, Giovana Arlene; LUI-NETTO, AdamoObjective: To describe the microbiological findings from bandage contact lenses in patients who work in a hospital environment submitted to photorefractive keratectomy (PRK). Methods: This prospective comparative case series enrolled 43 eyes of 22 volunteers (28.05 +/- 3.50 years). Fourteen individuals (n=27) were health care professionals who work in health care facilities or community physician's offices. Eight individuals (n=16) were patients who do not work in hospital environment. Photorefractive keratectomy was performed using standard technique, and a silicone hydrogel bandage contact lens was placed on the cornea and evaluated for adequate fit. Seven days after surgery, the bandage lenses were removed and imprinted in the following culture media: blood agar, chocolate agar, anaerobic-selective agar, and Sabouraud agar. When microbial growth was detected, the microorganism was identified, colony-forming units were quantified, and morphology and Gram-staining properties were analyzed. All isolates were tested for susceptibility to various antibiotics. Significance was assessed by Fisher exact test. Results: Microbial growth was detected in 16.27% of all contact lenses samples. No fungi or anaerobes were found. Microbial growth was only observed in bandage lenses removed from patients who work in hospital environments. Most microorganisms found were sensitive to all antibiotics tested. Conclusion: These results suggest that working in hospital environments increase contamination of the contact lenses after PRK.