GABRIEL RIBEIRO JUNIOR
Projetos de Pesquisa
Unidades Organizacionais
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
12 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 12
conferenceObject IMPACTS OF RECREATIONAL USE OF CANNABIS SATIVA ON GESTATIONAL AND DEVELOPMENTAL OUTCOMES IN MICE: PRELIMINARY RESULTS(2015) BENEVENUTO, S. G. M.; BELOTTI, L.; COSTA, N. S. X.; DOLHNIKOFF, M.; RIBEIRO JUNIOR, G.; LOURENCO, D. T.; VERAS, M. M.- Early and late pulmonary effects of nebulized LPS in mice: An acute lung injury model(2017) COSTA, Natalia de Souza Xavier; RIBEIRO JUNIOR, Gabriel; ALEMANY, Adair Aparecida dos Santos; BELOTTI, Luciano; ZATI, Douglas Hidalgo; CAVALCANTE, Marcela Frota; VERAS, Mariana Matera; RIBEIRO, Susan; KALLAS, Esper Georges; SALDIVA, Paulo Hilario Nascimento; DOLHNIKOTT, Marisa; SILVA, Luiz Fernando Ferraz daBackground and objective Acute respiratory distress syndrome (ARDS) has a high mortality rate of 35 - 46% depending on its severity. Animal models are crucial to better understand the pathophysiology of diseases, including ARDS. This study presents a feasible animal model of acute lung injury (ALI) using nebulized lipopolysaccharide (LPS) in a non-invasive approach, focusing on its short and long-term effects. Methods Mice received nebulized LPS or vehicle only (control group). Blood, BALF and lung tissue were collected 24 hours (LPS 24h) or 5 weeks (LPS 5w) after the nebulized LPS-induced lung injury. Inflammatory cytokines were assessed in the blood serum, BALF and lung tissue. Stereological analyses and remodeling changes were assessed by histology and immunohistochemistry at the specified time points. Results The LPS 24h group showed increased pro-inflammatory cytokine levels, intense cell influx, increased total septal volume, septal thickening and decreased surface density of the alveolar septa. The LPS 5w group showed persistent lung inflammation, septal thickening, increased total lung volume, accentuated collagen deposition, especially of collagen type I, and decreased MMP-2 protein expression. Conclusion We present a feasible, reproducible and non-invasive nebulized-LPS animal model that allows the assessment of both the acute and late phases of acute lung injury. The presence of lung remodeling with collagen deposition after 5 weeks makes it useful to study the pathophysiology, complications, and possible therapeutic intervention studies that aim to understand and reduce pulmonary fibrosis in the late phases of ALI.
conferenceObject Influence of exposure to concentrated urban air particles on the respiratory system of (LDLr-/-) mice(2014) MAUAD, Thais; ALEMANY, Adair; VERAS, Mariana; JUNIOR, Gabriel Ribeiro; COSTA, Natalia; CATANOZI, Sergio; ABDALLA, Dulcineia; SALDIVA, Paulo- Microplastics inhalation: evidence in human lung tissue(2021) AMATO-LOURENCO, Luis Fernando; OLIVEIRA, Regiani Carvalho; JUNIOR, Gabriel Ribeiro; GALVAO, Luciana Dos Santos; ANDO, Romulo Augusto; MAUAD, Thais
conferenceObject Particulate air pollution affects alveolization in a juvenile mice model of asthma(2014) VERAS, Mariana; TELES, Aila; COSTA, Natalia; RIBEIRO-JUNIOR, Gabriel; SALDIVA, Paulo; MAUAD, Thais- Stereological approach to the pathology of lung aging in humans(2020) SANTOS, Fabricio; FROIO, Francisca Lucas; RIBEIRO JUNIOR, Gabriel; VERAS, Mariana Matera; JUSTO, Lisie; SALDIVA, Paulo; COSTA, Natalia De Souza Xavier; MAUAD, Thais
conferenceObject SIRT1, SIRT2, SIRT6 and P21 gene expression in the lungs of mice chronically exposed to ambient particulate matter(2014) MAUAD, Thais; RIBEIRO, Gabriel; VERAS, Mariana; COSTA, Natalia; SHIMADA, Ana; ALEMANY, Adair; MENDES, Thais; SALDIVA, Paulo; FARSKY, Sandra- COVID-19 induces more pronounced extracellular matrix deposition than other causes of ARDS(2023) COSTA, Natalia de Souza Xavier; RIBEIRO JUNIOR, Gabriel; NASCIMENTO, Ellen Caroline Toledo do; BRITO, Jose Mara de; ANTONANGELO, Leila; FARIA, Caroline Silverio; MONTEIRO, Jhonatas Sirino; SETUBAL, Joao Carlos; PINHO, Joao Renato Rebello; PEREIRA, Roberta Verciano; SEELAENDER, Marilia; CASTRO, Gabriela Salim de; LIMA, Joanna D. C. C.; MONTEIRO, Renata Aparecida de Almeida; DUARTE-NETO, Amaro Nunes; SALDIVA, Paulo Hilario Nascimento; SILVA, Luiz Fernando Ferraz da; DOLHNIKOFF, Marisa; MAUAD, ThaisBackgroundLung fibrosis is a major concern in severe COVID-19 patients undergoing mechanical ventilation (MV). Lung fibrosis frequency in post-COVID syndrome is highly variable and even if the risk is proportionally small, many patients could be affected. However, there is still no data on lung extracellular matrix (ECM) composition in severe COVID-19 and whether it is different from other aetiologies of ARDS.MethodsWe have quantified different ECM elements and TGF-beta expression in lung tissue of 28 fatal COVID-19 cases and compared to 27 patients that died of other causes of ARDS, divided according to MV duration (up to six days or seven days or more). In COVID-19 cases, ECM elements were correlated with lung transcriptomics and cytokines profile.ResultsWe observed that COVID-19 cases presented significant increased deposition of collagen, fibronectin, versican, and TGF-beta, and decreased decorin density when compared to non-COVID-19 cases of similar MV duration. TGF-beta was precociously increased in COVID-19 patients with MV duration up to six days. Lung collagen was higher in women with COVID-19, with a transition of upregulated genes related to fibrillogenesis to collagen production and ECM disassembly along the MV course.ConclusionsFatal COVID-19 is associated with an early TGF-beta expression lung environment after the MV onset, followed by a disordered ECM assembly. This uncontrolled process resulted in a prominent collagen deposition when compared to other causes of ARDS. Our data provides pathological substrates to better understand the high prevalence of pulmonary abnormalities in patients surviving COVID-19.
- Diesel exhaust exposure and sirtuins gene expression in old mice(2017) RIBEIRO, Gabriel; COSTA, Natalia de Souza Xavier; BELOTTI, Luciano; ALEMANY, Adair; CUNHA, Paula Gabriela; DURO, Stephanie de Oliveira; MARCOURAKIS, Tania; FARSKY, Sandra; MAUAD, Thais
- Pulmonary remodeling after ARDS: a new experimental model(2019) GONCALVES, Cintia Tokio Reis; GONCALVES, Carlos Gustavo Oliveira Reis; COSTA, Natalia De S. X.; RIBEIRO, Gabriel; ASSIS, Edson F. De; SILVA, Luiz Fernando F. Da; CALDINI, Elia Garcia; FARIA-NETO, Hugo Caire C.; DOLHNIKOFF, Marisa