Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods

This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas.

Palavras-chave

Strongyloides stercoralis, molecular diagnosis, conventional PCR, real-time PCR

URI

https://observatorio.fm.usp.br/handle/OPI/9791

Referências

Goncalves ALR, 2011, REV SOC BRAS MED TRO, V44, P191, DOI [10.1590/S0037-86822011005000022, http://dx.doi.org/10.1590/s0037-86822011005000022]
Ines ED, 2011, ACTA TROP, V120, P206, DOI 10.1016/j.actatropica.2011.08.010
Khieu V, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002035
KOGA K, 1991, AM J TROP MED HYG, V45, P518
Lutz A, 1919, MEMORIAS I OSWALDO C, V11, P109
Mejia R, 2012, CURR OPIN INFECT DIS, V25, P458, DOI 10.1097/QCO.0b013e3283551dbd
Moghaddassani H, 2011, IRAN J PARASITOL, V6, P23
Olsen A, 2009, T ROY SOC TROP MED H, V103, P967, DOI 10.1016/j.trstmh.2009.02.013
Paula FM, 2011, PARASITOLOGY, V138, P1331, DOI 10.1017/S003118201100120X
RUGAI E., 1954, REV INST ADOLFO LUTZ, V14, P5
SAIKI RK, 1985, SCIENCE, V230, P1350, DOI 10.1126/science.2999980
Schar F, 2013, ACTA TROP, V126, P89, DOI 10.1016/j.actatropica.2012.12.012
ten Hove RJ, 2009, EUR J CLIN MICROBIOL, V28, P1045, DOI 10.1007/s10096-009-0745-1
Verweij JJ, 2009, T ROY SOC TROP MED H, V103, P342, DOI 10.1016/j.trstmh.2008.12.001

Coleções

Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - FM/MPR
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/06
Artigos e Materiais de Revistas Científicas - LIM/07

Página do item completo