LIM/46 - Laboratório de Parasitologia Médica
URI Permanente desta comunidade
O Laboratório de Parasitologia Médica é ligado ao Departamento de Moléstias Infecciosas e Parasitárias da Faculdade de Medicina da Universidade de São Paulo (FMUSP).
Linhas de pesquisa: parasitologia clínica (aplicada) envolvendo análises epidemiológicas, diagnóstico, tratamento (inclusive mediante uso de modelos experimentais) e prevenção; emprego de células-tronco no tratamento da miocardiopatia crônica da doença de Chagas e investigações sobre a Leishmaniose.
Site oficial: http://limhc.fm.usp.br/portal/lim46-laboratorio-de-parasitologia-medica/
Linhas de pesquisa: parasitologia clínica (aplicada) envolvendo análises epidemiológicas, diagnóstico, tratamento (inclusive mediante uso de modelos experimentais) e prevenção; emprego de células-tronco no tratamento da miocardiopatia crônica da doença de Chagas e investigações sobre a Leishmaniose.
Site oficial: http://limhc.fm.usp.br/portal/lim46-laboratorio-de-parasitologia-medica/
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- LIM/46 - Laboratório de Parasitologia Médica
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- LIM/46 - Laboratório de Parasitologia Médica
Submissões Recentes
Data-driven, cross-disciplinary collaboration: lessons learned at the largest academic health center in Latin America during the COVID-19 pandemic
(2024) RITTO, Ana Paula; ARAUJO, Adriana Ladeira de; CARVALHO, Carlos Roberto Ribeiro de; SOUZA, Heraldo Possolo De; FAVARETTO, Patricia Manga e Silva; SABOYA, Vivian Renata Boldrim; GARCIA, Michelle Louvaes; KULIKOWSKI, Leslie Domenici; KALLAS, Esper Georges; PEREIRA, Antonio Jose Rodrigues; COBELLO JUNIOR, Vilson; SILVA, Katia Regina; ABDALLA, Eidi Raquel Franco; SEGURADO, Aluisio Augusto Cotrim; SABINO, Ester Cerdeira; RIBEIRO JUNIOR, Ulysses; FRANCISCO, Rossana Pulcineli Vieira; MIETHKE-MORAIS, Anna; LEVIN, Anna Sara Shafferman; SAWAMURA, Marcio Valente Yamada; FERREIRA, Juliana Carvalho; SILVA, Clovis Artur; MAUAD, Thais; GOUVEIA, Nelson da Cruz; LETAIF, Leila Suemi Harima; BEGO, Marco Antonio; BATTISTELLA, Linamara Rizzo; DUARTE, Alberto Jose da Silva; SEELAENDER, Marilia Cerqueira Leite; MARCHINI, Julio; FORLENZA, Orestes Vicente; ROCHA, Vanderson Geraldo; MENDES-CORREA, Maria Cassia; COSTA, Silvia Figueiredo; CERRI, Giovanni Guido; BONFA, Eloisa Silva Dutra de Oliveira; CHAMMAS, Roger; BARROS FILHO, Tarcisio Eloy Pessoa de; BUSATTO FILHO, Geraldo
Introduction The COVID-19 pandemic has prompted global research efforts to reduce infection impact, highlighting the potential of cross-disciplinary collaboration to enhance research quality and efficiency.Methods At the FMUSP-HC academic health system, we implemented innovative flow management routines for collecting, organizing and analyzing demographic data, COVID-related data and biological materials from over 4,500 patients with confirmed SARS-CoV-2 infection hospitalized from 2020 to 2022. This strategy was mainly planned in three areas: organizing a database with data from the hospitalizations; setting-up a multidisciplinary taskforce to conduct follow-up assessments after discharge; and organizing a biobank. Additionally, a COVID-19 curated collection was created within the institutional digital library of academic papers to map the research output.Results Over the course of the experience, the possible benefits and challenges of this type of research support approach were identified and discussed, leading to a set of recommended strategies to enhance collaboration within the research institution. Demographic and clinical data from COVID-19 hospitalizations were compiled in a database including adults and a minority of children and adolescents with laboratory confirmed COVID-19, covering 2020-2022, with approximately 350 fields per patient. To date, this database has been used in 16 published studies. Additionally, we assessed 700 adults 6 to 11 months after hospitalization through comprehensive, multidisciplinary in-person evaluations; this database, comprising around 2000 fields per subject, was used in 15 publications. Furthermore, thousands of blood samples collected during the acute phase and follow-up assessments remain stored for future investigations. To date, more than 3,700 aliquots have been used in ongoing research investigating various aspects of COVID-19. Lastly, the mapping of the overall research output revealed that between 2020 and 2022 our academic system produced 1,394 scientific articles on COVID-19.Discussion Research is a crucial component of an effective epidemic response, and the preparation process should include a well-defined plan for organizing and sharing resources. The initiatives described in the present paper were successful in our aim to foster large-scale research in our institution. Although a single model may not be appropriate for all contexts, cross-disciplinary collaboration and open data sharing should make health research systems more efficient to generate the best evidence.
Thinking out of the box: revisiting health surveillance based on medical records
(2023) SAMPAIO, V. S.; LOPES, R.; OZAHATA, M. C.; NAKAYA, H. I.; SOUSA, E.; ARAúJO, J. D.; BRAGATTE, M. A. S.; BRITO, A. F.; GRESPAN, R. M. Z.; CAPUANI, M. L. D.; DOMINGUES, H. H.; PELLINI, A. C. G.; MATEOS, S. D. O. G.; CONDE, M. T. R. P.; LEAL, F. Eudes; SABINO, E.; SIMãO, M.; KALIL, J.
Despite the considerable advances in the last years, the health information systems for health surveillance still need to overcome some critical issues so that epidemic detection can be performed in real time. For instance, despite the efforts of the Brazilian Ministry of Health (MoH) to make COVID-19 data available during the pandemic, delays due to data entry and data availability posed an additional threat to disease monitoring. Here, we propose a complementary approach by using electronic medical records (EMRs) data collected in real time to generate a system to enable insights from the local health surveillance system personnel. As a proof of concept, we assessed data from São Caetano do Sul City (SCS), São Paulo, Brazil. We used the fever term as a sentinel event. Regular expression techniques were applied to detect febrile diseases. Other specific terms such as malaria, dengue, Zika, or any infectious disease were included in the dictionary and mapped to fever. Additionally, after tokenizing, we assessed the frequencies of most mentioned terms when fever was also mentioned in the patient complaint. The findings allowed us to detect the overlapping outbreaks of both COVID-19 Omicron BA.1 subvariant and Influenza A virus, which were confirmed by our team by analyzing data from private laboratories and another COVID-19 public monitoring system. Timely information generated from EMRs will be a very important tool to the decision-making process as well as research in epidemiology. Quality and security on the data produced is of paramount importance to allow the use by health surveillance systems.
Rapid viral metagenomics using SMART-9N amplification and nanopore sequencing
(2023) CLARO, I. M.; RAMUNDO, M. S.; COLETTI, T. M.; SILVA, C. A. M. da; VALENCA, I. N.; CANDIDO, D. S.; SALES, F. C. S.; MANULI, E. R.; JESUS, J. G. de; PAULA, A. de; FELIX, A. C.; ANDRADE, P. D. S.; PINHO, M. C.; SOUZA, W. M.; AMORIM, M. R.; PROENCA-MODENA, J. L.; KALLAS, E. G.; LEVI, J. E.; FARIA, N. R.; SABINO, E. C.; LOMAN, N. J.; QUICK, J.
Emerging and re-emerging viruses are a global health concern. Genome sequencing as an approach for monitoring circulating viruses is currently hampered by complex and expensive methods. Untargeted, metagenomic nanopore sequencing can provide genomic information to identify pathogens, prepare for or even prevent outbreaks. SMART (Switching Mechanism at the 5′ end of RNA Template) is a popular approach for RNA-Seq but most current methods rely on oligo-dT priming to target polyadenylated mRNA molecules. We have developed two random primed SMART-Seq approaches, a sequencing agnostic approach ‘SMART-9N’ and a version compatible rapid adapters available from Oxford Nanopore Technologies ‘Rapid SMART-9N’. The methods were developed using viral isolates, clinical samples, and compared to a gold-standard amplicon-based method. From a Zika virus isolate the SMART-9N approach recovered 10kb of the 10.8kb RNA genome in a single nanopore read. We also obtained full genome coverage at a high depth coverage using the Rapid SMART-9N, which takes only 10 minutes and costs up to 45% less than other methods. We found the limits of detection of these methods to be 6 focus forming units (FFU)/mL with 99.02% and 87.58% genome coverage for SMART-9N and Rapid SMART-9N respectively. Yellow fever virus plasma samples and SARS-CoV-2 nasopharyngeal samples previously confirmed by RT-qPCR with a broad range of Ct-values were selected for validation. Both methods produced greater genome coverage when compared to the multiplex PCR approach and we obtained the longest single read of this study (18.5 kb) with a SARS-CoV-2 clinical sample, 60% of the virus genome using the Rapid SMART-9N method. This work demonstrates that SMART-9N and Rapid SMART-9N are sensitive, low input, and long-read compatible alternatives for RNA virus detection and genome sequencing and Rapid SMART-9N improves the cost, time, and complexity of laboratory work.
Genetic variation in HLA genes: impact on transplant compatibility in a Brazilian admixed population
(2023) ANDRADE, Heloisa S.; SILVA, Marcio; NUNES, Kelly; PASSOS, Carlos Henrique; SENA, Alexandre C.; CASTELLI, Erick C.; DINARDO, Carla; SABINO, Ester C.; TEIXEIRA, Carolina M.; TELES, Dahra; AMORIM, Luiz; CUSTER, Brian; KELLY, Shannon; PORTO, Luis Cristovao; MEYER, Diogo
Value of left ventricular longitudinal strain in predicting overt heart failure in patients with Chagas disease and preserved left ventricular ejection fraction
(2023) NUNES, M. C. P. N.; FERREIRA, A. M. F.; SILVA, J. L. P. S.; CARVALHO, V. T. C.; SANTOS-JUNIOR, O. R. S. J.; CARDOSO, C. S. C.; OLIVEIRA, L. C. O.; SANTOS, A. C. J. S.; CRUZ, D. S. C.; LEITE, S. F. L.; JESUS, A. P. J.; HAIKAL, D. S. A. H.; SABINO, E. C. S.; RIBEIRO, A. L. P. R.
Exploring the prognostic significance and important phenotypic and genotypic associations of neural network-derived electrocardiographic features
(2023) SAU, Arunashis; GIATTI, Luana; NG, Fu Siong; PETERS, Nicholas; SHIPLEY, Martin; BARRETO, Sandhi; PASTIKA, Libor; RIBEIRO, Antonio; SABINO, Ester; WARE, James; MCGURK, Kathryn; ANDRSOVA, Irena; WU, Huiyi; MALIK, Marek; RIBEIRO, Antonio; ZHENG, Sean; O'REGAN, Declan; WAKS, Jonathan; ARDISSINO, Maddalena; BAJAJ, Nikesh; HNATKOVA, Katerina; SHI, Xili; MANDIC, Danilo; KRAMER, Daniel; BRITTON, Annie; CHEN, Jun Yu; NOVOTNY, Tomas
Recognition of Trypanosoma cruzi epitopes by IgG of benznidazole treated Chagas disease patients
(2021) CARNERO, Luis Antonio Rodriguez; MONTEIRO, Jhonatas Sirino; SETUBAL, Joao Carlos; SABINO, Ester Cerdeira; NETO, Edecio Cunha; GIORDANO, Ricardo Jose
Introduction, Dispersal, and Predominance of SARS-CoV-2 Delta Variant in Rio Grande do Sul, Brazil: A Retrospective Analysis
(2023) CASTRO, Thais Regina y; PICCOLI, Bruna C.; VIEIRA, Andressa A.; CASARIN, Bruna C.; TESSELE, Luiza F.; SALVATO, Richard S.; GREGIANINI, Tatiana S.; MARTINS, Leticia G.; RESENDE, Paola Cristina; PEREIRA, Elisa C.; MOREIRA, Filipe R. R.; JESUS, Jaqueline G. de; SEERIG, Ana Paula; LOBATO, Marcos Antonio O.; CAMPOS, Marli M. A. de; GOULARTE, Juliana S.; SILVA, Mariana S. da; DEMOLINER, Meriane; FILIPPI, Micheli; PEREIRA, Vyctoria M. A. Goes; SCHWARZBOLD, Alexandre V.; SPILKI, Fernando R.; TRINDADE, Priscila A.
Mutations in the SARS-CoV-2 genome can alter the virus' fitness, leading to the emergence of variants of concern (VOC). In Brazil, the Gamma variant dominated the pandemic in the first half of 2021, and from June onwards, the first cases of Delta infection were documented. Here, we investigate the introduction and dispersal of the Delta variant in the RS state by sequencing 1077 SARS-CoV-2-positive samples from June to October 2021. Of these samples, 34.7% were identified as Gamma and 65.3% as Delta. Notably, 99.2% of Delta sequences were clustered within the 21J lineage, forming a significant Brazilian clade. The estimated clock rate was 5.97 x 10-4 substitutions per site per year. The Delta variant was first reported on 17 June in the Vinhedos Basalto microregion and rapidly spread, accounting for over 70% of cases within nine weeks. Despite this, the number of cases and deaths remained stable, possibly due to vaccination, prior infections, and the continued mandatory mask use. In conclusion, our study provides insights into the Delta variant circulating in the RS state, highlighting the importance of genomic surveillance for monitoring viral evolution, even when the impact of new variants may be less severe in a given region.
Nanobiotics and the One Health Approach: Boosting the Fight against Antimicrobial Resistance at the Nanoscale
(2023) Himanshu; MUKHERJEE, Riya; VIDIC, Jasmina; LEAL, Elcio; COSTA, Antonio Charlys da; PRUDENCIO, Carlos Roberto; RAJ, V. Samuel; CHANG, Chung-Ming; PANDEY, Ramendra Pati
Antimicrobial resistance (AMR) is a growing public health concern worldwide, and it poses a significant threat to human, animal, and environmental health. The overuse and misuse of antibiotics have contributed significantly and others factors including gene mutation, bacteria living in biofilms, and enzymatic degradation/hydrolyses help in the emergence and spread of AMR, which may lead to significant economic consequences such as reduced productivity and increased health care costs. Nanotechnology offers a promising platform for addressing this challenge. Nanoparticles have unique properties that make them highly effective in combating bacterial infections by inhibiting the growth and survival of multi-drug-resistant bacteria in three areas of health: human, animal, and environmental. To conduct an economic evaluation of surveillance in this context, it is crucial to obtain an understanding of the connections to be addressed by several nations by implementing national action policies based on the One Health strategy. This review provides an overview of the progress made thus far and presents potential future directions to optimize the impact of nanobiotics on AMR.
Estimated glomerular filtration rate in Brazilian adults with sickle cell disease: results from the REDS-III multicenter cohort study
(2023) BELISARIO, Andre Rolim; SILVA, Ana Cristina Simoes e; MOURA, Isabel Cristina Gomes; CARNEIRO-PROIETTI, Anna Barbara; SABINO, Ester Cerdeira; LOUREIRO, Paula; MAXIMO, Claudia; FLOR-PARK, Miriam V.; RODRIGUES, Daniela de Oliveira Werneck; OZAHATA, Mina Cintho; MOTA, Rosimere Afonso; DINARDO, Carla Luana; KELLY, Shannon; CUSTER, Brian
Chronic kidney disease (CKD) has a significant impact on sickle cell disease (SCD) morbidity and mortality. Early identification of individuals at highest risk of developing CKD may allow therapeutic intervention to prevent worse outcomes. This study aimed to evaluate the prevalence and risk factors for reduced estimated glomerular filtration rate (eGFR) among adults with SCD in Brazil. Participants in the REDS-III multicenter SCD cohort with more severe genotypes aged >= 18 years with at least two serum creatinine values were analyzed. The eGFR was calculated using the Jamaica Sickle Cell Cohort Study GFR equation. The eGFR categories were defined according to the K/DOQI. Participants with eGFR >= 90 were compared to those with those with eGFR < 90. Among the 870 participants, 647 (74.4%) had eGFR >= 90, 211 (24.3%) had eGFR 60 to 89, six (0.7%) had eGFR 30 to 59, and six (0.7%) had ESRD. Male sex (OR: 37.3; 95%CI: 22.4-65.1), higher age (OR: 1.04; 95%CI: 1.02-1.06), higher diastolic blood pressure (OR: 1.03; 95%CI: 1.009-1.06), lower Hb (OR: 0.80; 95%CI: 0.68-0.93), and lower reticulocytes (OR: 0.94; 95%CI: 0.89-0.99) levels were independently associated with eGFR < 90. There was a trend towards higher odds of death in participants with eGFR < 90 (OR: 1.8; 95%CI: 0.95-3.32; p = 0.065). In turn, participants with eGFR < 60 had a 12.2 (95%CI: 2.1-96.9) times higher odds for death when compared to those with eGFR >= 60. In this study, eGFR < 90 was observed in one-quarter of adults. Older age, male sex, higher diastolic blood pressure, lower hemoglobin, and lower reticulocyte levels were associated with occurrence of eGFR < 90. Estimated GFR < 60 increased the risk of mortality.