LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses

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https://observatorio.fm.usp.br/handle/OPI/3618
O Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses é ligado ao Departamento de Doenças Infecciosas e Parasitárias da Faculdade de Medicina da Universidade de São Paulo (FMUSP).

Linhas de pesquisa: esquistossomose, toxocaríase, estrongiloidíase, blastocistose: epidemiologia, diagnóstico; leishmanioses; aspectos histopatológicos e estudo da resposta imune tecidual; AIDS: aspectos histopatológicos e estudo da compartimentalização da resposta imune; febres hemorrágicas: estudo da patogenia e técnicas diagnósticas; mucosa oral e neoplasias dermatológicas: estudos morfológicos e moleculares; leptospirose: patologia renal; hepatites autoimunes, virais e hepatopatias metabólicas.

Site oficial: http://limhc.fm.usp.br/portal/lim06-laboratorio-de-imunopatologia-da-esquistossomose-e-outras-parasitoses/

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Leptospiroses
(2021) DIAMENT, Décio; LOMAR, André Villela; BRITO, Thales de; ROMERO, Eliete Caló
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Expression patterns of Interleukin-33 and its receptor ST2 in severe COVID-19
(2023) LINDO, Caroline; COSTA, Natalia De Souza Xavier; SIDDHURAJ, Premkumar; JONSSON, Jimmie; ORENGO, Jamie M.; SLEEMAN, Matthew A.; LINDSTEDT, Sandra; ANDERSSON, Cecilia; SILVA, Luiz Fernando; DUARTENETO, Armaro Nunes; SALDIVA, Paulo Hilario Nascimento; SANDEN, Caroline; MAUAD, Thais; ERJEFALT, Jonas
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Lung ECM composition, its influence factors and transcriptomics in the lungs of severe COVID-19.
(2023) COSTA, Natalia de Souza Xavier; RIBEIRO JUNIOR, Gabriel; NASCIMENTO, Ellen Toledo Do; BRITO, Jose Mara De; MONTEIRO, Jhonatas Sirino; SETUBAL, Joao Carlos; PINHO, Joao Renato Rebello; PEREIRA, Roberta Verciano; MONTEIRO, Renata Aparecida De Almeida; DUARTE NETO, Amaro Nunes; SALDIVA, Paulo Hilario Nascimento; SILVA, Luiz Fernando Ferraz Da; DOLHNIKOFF, Marisa; MAUAD, Thais
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microRNAs associated with metastatic potential in salivary gland mucoepidermoid carcinoma
(2023) TREVIZANI, Maria Eduarda S.; OLIVEIRA, Katia K.; MARCHI, Fabio A.; BIZINELLI, Daniela; MARIANO, Fernanda V.; NAGANO, Cibele P.; COSTA, Felipe A.; PINTO, Clovis A.; KOWALSKI, Luiz P.; LOURENCO, Silvia V.; COUTINHO-CAMILLO, Claudia M.
article 3 Citação(ões) na Scopus
Enhancing the antischistosomal activity of carvacryl acetate using nanoemulsion
(2023) SOUZA, Rafael L. de; MENGARDA, Ana C.; ROQUINI, Daniel B.; MELO, Camila O.; MORAIS, Mayara C. de; ESPIRITO-SANTO, Maria Cristina; SOUSA, Damiao P. de; MORAES, Josue de; OLIVEIRA, Elquio E.
Aim: To formulate a carvacryl acetate nanoemulsion (CANE) and test its antischistosomal activity. Materials & methods: CANE was prepared and tested in vitro on Schistosoma mansoni adult worms and both human and animal cell lines. Next, CANE was administered orally to mice infected with either a prepatent infection or a patent infection of S. mansoni. Results: CANE was stable during 90 days of analysis. CANE showed in vitro anthelmintic activity, and no cytotoxic effects were observed. In vivo, CANE was more effective than the free compounds in reducing worm burden and egg production. Treatment with CANE was more effective for prepatent infections than praziquantel. Conclusion: CANE improves antiparasitic properties and may be a promising delivery system for schistosomiasis treatment.
article 0 Citação(ões) na Scopus
COVID-19: detection methods in post-mortem samples
(2023) TEDESCO, Ilaria; MARINO, Federica Zito; RONCHI, Andrea; NETO, Amaro Nunes Duarte; DOLHNIKOFF, Marisa; MUNICINO, Maurizio; CAMPOBASSO, Carlo Pietro; PANNONE, Giuseppe; FRANCO, Renato
COVID-19 identification is routinely performed on fresh samples, such as nasopharyngeal and oropharyngeal swabs, even if, the detection of the virus in formalin-fixed paraffinembedded (FFPE) autopsy tissues could help to underlie mechanisms of the pathogenesis that are not well understood. The gold standard for COVID-19 detection in FFPE samples remains the qRT-PCR as in swab samples, contextually other methods have been developed, including immunohistochemistry (IHC), and in situ hybridization (ISH). In this manuscript, we summarize the main data regarding the methods of COVID-19 detection in pulmonary and extra-pulmonary post-mortem samples, and especially the sensitivity and specificity of these assays will be discussed.
article 1 Citação(ões) na Scopus
Monkeypox Virus Immune Evasion and Eye Manifestation: Beyond Eyelid Implications
(2023) LUCENA-NETO, Francisco D.; FALCAO, Luiz F. M.; VIEIRA-JUNIOR, Adolfo S.; MORAES, Evelly C. S.; DAVID, Joacy P. F.; SILVA, Camilla C.; SOUSA, Jorge R.; DUARTE, Maria I. S.; VASCONCELOS, Pedro F. C.; QUARESMA, Juarez A. S.
Monkeypox virus (MPXV), belonging to the Poxviridae family and Orthopoxvirus genus, is closely related to the smallpox virus. Initial prodromal symptoms typically include headache, fever, and lymphadenopathy. This review aims to detail various ocular manifestations and immune evasion associated with the monkeypox viral infection and its complications, making it appropriate as a narrative review. Common external ocular manifestations of MPXV typically involve a generalized pustular rash, keratitis, discharges, and dried secretions related to conjunctival pustules, photophobia, and lacrimation. Orthopoxviruses can evade host immune responses by secreting proteins that antagonize the functions of host IFN gamma, CC and CXC chemokines, IL-1 beta, and the complement system. One of the most important transcription factors downstream of pattern recognition receptors binding is IRF3, which controls the expression of the crucial antiviral molecules IFN alpha and IFN beta. We strongly recommend that ophthalmologists include MPXV as part of their differential diagnosis when they encounter similar cases presenting with ophthalmic manifestations such as conjunctivitis, blepharitis, or corneal lesions. Furthermore, because non-vaccinated individuals are more likely to exhibit these symptoms, it is recommended that healthcare administrators prioritize smallpox vaccination for at-risk groups, including very young children, pregnant women, older adults, and immunocompromised individuals, especially those in close contact with MPXV cases.
article 1 Citação(ões) na Scopus
Immunohistochemical Characterization of M1, M2, and M4 Macrophages in Leprosy Skin Lesions
(2023) QUARESMA, Tatiane Costa; VALENTIM, Livia de Aguiar; SOUSA, Jorge Rodrigues de; AARAO, Tinara Leila de Souza; FUZII, Hellen Thais; DUARTE, Maria Irma Seixas; SOUZA, Juarez de; QUARESMA, Juarez Antonio Simoes
Mycobacterium leprae is the etiological agent of leprosy. Macrophages (M phi s) are key players involved in the pathogenesis of leprosy. In this study, immunohistochemical analysis was performed to examine the phenotype of M phi subpopulations, namely M1, M2, and M4, in the skin lesions of patients diagnosed with leprosy. Based on the database of treatment-na & iuml;ve patients treated between 2015 and 2019 at the Department of Dermatology of the University of the State of Par & aacute;, Bel & eacute;m, routine clinical screening samples were identified. The monolabeling protocol was used for M1 macrophages (iNOS, IL-6, TNF-alpha) and M2 macrophages (IL-10, IL-13, CD163, Arginase 1, TGF-beta, FGFb), and the double-labeling protocol was used for M4 macrophages (IL-6, MMP7, MRP8, TNF-alpha e CD68). To confirm the M4 macrophage lineage, double labeling of the monoclonal antibodies CD68 and MRP8 was also performed. Our results demonstrated a statistically significant difference for the M1 phenotype among the Virchowian (VV) (4.5 +/- 1.3, p < 0.0001), Borderline (1.6 +/- 0.4, p < 0.0001), and tuberculoid (TT) (12.5 +/- 1.8, p < 0.0001) clinical forms of leprosy. Additionally, the M2 phenotype showed a statistically significant difference among the VV (12.5 +/- 2.3, p < 0.0001), Borderline (1.3 +/- 0.2, p < 0.0001), and TT (3.2 +/- 0.7, p < 0.0001) forms. For the M4 phenotype, a statistically significant difference was observed in the VV (9.8 +/- 1.7, p < 0.0001), Borderline (1.2 +/- 0.2, p < 0.0001), and TT (2.6 +/- 0.7, p < 0.0001) forms. A significant correlation was observed between the VV M1 and M4 (r = 0.8712; p = 0.0000) and between the VV M2 x TT M1 (r = 0.834; p = 0.0002) phenotypes. The M1 M phi s constituted the predominant M phi subpopulation in the TT and Borderline forms of leprosy, whereas the M2 M phi s showed increased immunoexpression and M4 was the predominant M phi phenotype in VV leprosy. These results confirm the relationship of the M phi profile with chronic pathological processes of the inflammatory response in leprosy.
article 0 Citação(ões) na Scopus
Intranasal Liposomal Formulation of Spike Protein Adjuvanted with CpG Protects and Boosts Heterologous Immunity of hACE2 Transgenic Mice to SARS-CoV-2 Infection
(2023) RUSSO, Momtchilo; MENDES-CORREA, Maria Cassia; LINS, Bruna B.; KERSTEN, Victor; PERNAMBUCO, Paulo C. A.; MARTINS, Toni Ricardo; TOZETTO-MENDOZA, Tania Regina; BOAS, Lucy Santos Vilas; GOMES, Brisa Moreira; DATI, Livia Mendonca Munhoz; DUARTE-NETO, Amaro Nunes; REIGADO, Gustavo Roncoli; FREDERICO, Ana Beatriz T.; CUNHA, Danielle R. de A.; PAULA, Anderson Vicente de; SILVA, Jose Igor G. da; VASCONCELOS, Carlos F. Moreira; CHAMBERGO, Felipe S.; NUNES, Viviane Abreu; BOM, Ana Paula Dinis Ano; CASTILHO, Leda R.; MARTINS, Rodrigo A. P.; HIRATA, Mario Hiroyuki; MIROTTI, Luciana; TRIPP, Ralph A.
Mucosal vaccination appears to be suitable to protect against SARS-CoV-2 infection. In this study, we tested an intranasal mucosal vaccine candidate for COVID-19 that consisted of a cationic liposome containing a trimeric SARS-CoV-2 spike protein and CpG-ODNs, a Toll-like receptor 9 agonist, as an adjuvant. In vitro and in vivo experiments indicated the absence of toxicity following the intranasal administration of this vaccine formulation. First, we found that subcutaneous or intranasal vaccination protected hACE-2 transgenic mice from infection with the wild-type (Wuhan) SARS-CoV-2 strain, as shown by weight loss and mortality indicators. However, when compared with subcutaneous administration, the intranasal route was more effective in the pulmonary clearance of the virus and induced higher neutralizing antibodies and anti-S IgA titers. In addition, the intranasal vaccination afforded protection against gamma, delta, and omicron virus variants of concern. Furthermore, the intranasal vaccine formulation was superior to intramuscular vaccination with a recombinant, replication-deficient chimpanzee adenovirus vector encoding the SARS-CoV-2 spike glycoprotein (Oxford/AstraZeneca) in terms of virus lung clearance and production of neutralizing antibodies in serum and bronchial alveolar lavage (BAL). Finally, the intranasal liposomal formulation boosted heterologous immunity induced by previous intramuscular vaccination with the Oxford/AstraZeneca vaccine, which was more robust than homologous immunity.
article 1 Citação(ões) na Scopus
Strongyloides infection screening in transplant candidates: What is the best strategy?
(2023) GRYSCHEK, Ronaldo Cesar Borges; CORRAL, Marcelo Andreetta; SITTA, Renata Barnabe; GOTTARDI, Maiara; PIERROTTI, Ligia Camera; COSTA, Silvia Figueiredo; ABDALA, Edson; CHIEFFI, Pedro Paulo; PAULA, Fabiana Martins de
Background: The potential that Strongyloides stercoralis infection has to cause major morbidity and high mortality when the disseminated form occurs in transplant patients is of particular concern.Methods: In this study, the objective was to observe S. stercoralis infection in patients who are candidates for transplantation by using parasitological, serological, and molecular techniques and to propose an algorithm for the detection of that infection in transplant candidates.Results: By parasitological techniques, 10% of fecal samples were positive. Anti-Strongyloides antibodies immunoglobulin G were detected in 19.3% and 20.7% of patients by immunofluorescence assay and enzyme-linked immunosorbent assay, respectively. S. stercoralis DNA was observed in 17.3% of samples by conventional polymerase chain reaction and 32.7% of samples by quantitative polymerase chain reaction (qPCR).Conclusion: The set of results allows us to reinforce that a positive result by parasitological techniques and/or qPCR indicates that the specific treatment should be applied. However, the improvement of diagnostic techniques may suggest changes in the screening for strongyloidiasis in these patients. image