Laboratórios de Investigação Médica - LIMs

URI Permanente desta comunidade

https://observatorio.fm.usp.br/handle/OPI/3597
Os Laboratórios de Investigação Médica (LIMs) foram criados em decorrência da Reforma Universitária de 1969 (Lei de Diretrizes e Bases da Educação Nacional), implantada em 1970, através da qual os laboratórios de pesquisa passaram a ocupar área física da Faculdade e a receber materiais e recursos humanos do Hospital das Clínicas. Pelo Decreto 9720 de 20 de abril de 1977, o Hospital das Clínicas FMUSP incorporou os Laboratórios de Investigação Médica.

Os LIMs abrigam mais de 200 grupos de pesquisa, que são acadêmica e cientificamente vinculados aos Departamentos da FMUSP e administrativamente vinculados ao HC-FMUSP; possuindo as seguintes finalidades: desenvolver pesquisa científica; padronizar novas técnicas e métodos de diagnóstico possibilitando sua implantação para atendimento de pacientes em outras unidades do HC; promover a formação de pesquisadores em pesquisa básica e aplicada; servir de campo de ensino e treinamento de estudantes de escolas de nível superior cujos currículos sejam relacionados com as ciências da saúde; servir de campo de desenvolvimento e treinamento para profissionais da saúde e realizar cursos no campo da medicina e da saúde.

Site oficial: http://lims.fm.usp.br/

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article
Dry needling in the treatment of myofascial trigger points and painful shoulder dysfunctions: narrative review of the literature
(2023) ANTôNIO, Jhonatan Zimmermann; MATIELO, Heloísa Alonso; MINSON, Fabíola; DALE, Camila Squarzoni
ABSTRACT BACKGROUND AND OBJECTIVES: Shoulder painful dysfunctions comprises one of the most common musculoskeletal disorders that requires specialized assistance. Dry Needling (DN) became an adjuvant approach with increased use in clinical practice to treat this type of condition. The present study discusses the literature related to DN in the treatment of myofascial trigger points (MTPs), shoulder dysfunctions and associated pain. METHODS: A narrative review through search of articles from 2010 to 2022 written in Portuguese, English or Spanish was performed in Latin American and Caribbean Literature on Health Sciences (LILACS), Health Information from the National Library of Medicine (Medline), Web of Science and the Scientific Electronic Library Online (Scielo) databases using the keywords: <“Dry Needling”>; <“Agulhamento a Seco”>; <“Myofascial Trigger Points”>; <“Pontos-Gatilhos Miofasciais”>; <“Shoulder Dysfunctions”>; <”Disfunções do ombro”>. The qualitative analysis was performed determining the level of evidence for DN treatment of MTPs, shoulder dysfunctions and pain. RESULTS: A total of 45 citations were found, 22 citations were excluded because they did not meet the selection criteria. The 23 remaining citations were examined for titles and abstracts and duplicate studies were removed. Finally, 10 articles met the selection criteria and were included in the present review. No articles were excluded after full-text screening. The analysis showed poor advances and knowledge regarding the application of DN for the treatment of pain, painful and general shoulder dysfunctions and MTPs, with few evidence regarding treatment effectiveness, patient’s pain scores data, mechanisms of action and statistical analysis. CONCLUSION: There is still a lack of concrete scientific evidence to assess DN effectiveness in modulating pain in patients with MTPs shoulder. More systematic reviews and meta-analyses together with experimental and clinical searches must be conducted to provide stronger evidence of this modality to relief painful symptoms in the shoulder, as well as a treatment of MTPs and general shoulder disorders.
bookPart
Dor lombar
(2022) KOBAYASHI, Ricardo; HERRERO, Fernando; ISHI, Marcos Masayuki
bookPart
Diagnóstico laboratorial e radiológico
(2021) GIANNINI, Maria José Soares Mendes; NEGRO, Gilda Maria Barbaro Del; GONçALVES, Elmar Gonzaga
bookPart
Infecção pelo vírus Epstein-Barr (EBV) - mononucleose infecciosa
(2021) FINK, Maria Cristina Domingues da Silva; MENDOZA, Tania Regina Tozetto; BRAZ-SILVA, Paulo Henrique
bookPart
A captação de recursos através de agências governamentais
(2023) FACANALI JUNIOR, Marcio Roberto; ARTIFON, Everson Luiz Almeida
bookPart
Judicialização nos processos das emendas parlamentares e outros recursos da saúde
(2023) MATHEUS, Sidney; ARTIFON, Everson Luiz Almeida
bookPart
Leptospiroses
(2021) DIAMENT, Décio; LOMAR, André Villela; BRITO, Thales de; ROMERO, Eliete Caló
article 0 Citação(ões) na Scopus
Human interleukin-36γ plays a crucial role in cytokine induction during Sporothrix brasiliensis and S. schenckii infection in keratinocytes and PBMCs
(2024) KISCHKEL, Brenda; SANTOS, Jessica C. dos; LOPES-BEZERRA, Leila; TABORDA, Carlos P.; JOOSTEN, Leo A. B.
Cytokines of the interleukin (IL)-1 superfamily including the different IL-36 isoforms, have been reported as mediators of acute and chronic inflammation in human skin diseases, such as psoriasis. Here, we demonstrated for the first time that Sporothrix schenckii and S. brasiliensis, the fungi that cause subcutaneous infection sporotrichosis, can induce the expression of IL-36 alpha, IL-36 gamma and IL-36Ra in human keratinocytes and primary peripheral blood mononuclear cells (PBMCs). Specifically, IL-36 gamma was differentially expressed by keratinocytes stimulated with Sporothrix yeasts when compared to the commensal microorganism Staphylococcus epidermidis. The exposure of keratinocytes to 24 h or 7-days culture supernatant of PBMCs stimulated with Sporothrix induced higher IL-36 gamma production compared to direct stimulation of keratinocytes with the live fungus. We identified that IL-36 gamma mRNA expression in keratinocytes is increased in the presence of IL-17, TNF, IL-113 and IL-1 alpha and these cytokines may act synergistically to maintain IL-36 gamma production. Lastly, using a cohort of 164 healthy individuals, we showed that individuals carrying variants of the IL36G gene (rs11690399 and rs11683399) exhibit increased IL-36 gamma production as well as increased innate cytokine production after Sporothrix exposure. Importantly, stimulation of PBMCs with recombinant IL-36 gamma increased the production of IL-113 and IL-6, while IL-36Ra were able to decrease the concentration of these cytokines. Our findings contribute to the understanding of the pathogenesis of sporotrichosis and suggest that IL-36 gamma may be involved in maintaining the cytokine loop that leads to tissue destruction by exacerbating the immune response in sporotrichosis. Of high interest, we present the IL-36 signalling pathway as a potential new therapeutic target.
article 1 Citação(ões) na Scopus
EZH2 Expression Correlates With T-Cell Infiltration in Oral Leukoplakia and Predicts Cancer Transformation
(2023) GANESH, Divya; DAFAR, Amal; NIKLASSON, Julia; SANDBERG, Ingrid; BRAZ-SILVA, Paulo; SAPKOTA, Dipak; OHMAN, Jenny; GIGLIO, Daniel; HASSEUS, Bengt
Background/Aim: The EZH2 complex is involved in cellular proliferation and modulates the immune response in cancer. Less is known about the importance of EZH2 in precancerous lesions such as oral leukoplakia (OL). The aim of the study was to explore the association between EZH2 expression, immune activation, and cancer transformation in OL. Patients and Methods: Analyses were retrospectively performed on nine OL cases that had undergone transformation to oral squamous cell carcinoma (OSCC; OL-ca) and nine that had not undergone transformation (OL-non). EZH2-expressing cells, CD3+ and CD8+ T cells, and CD1a+ Langerhans cells were visualized with immunohistofluorescence and counted. Results: A moderate positive correlation between CD3-and EZH2-expressing and CD8-and EZH2-expressing cells in the epithelium was found (r=0.57, p=0.01; r=0.59, p=0.01). The number of EZH2-expressing cells in the epithelium of OL-ca was significantly higher compared to OL-non (p=0.0002). Cancer - free survival rates differed significantly between patients with EZH2high compared to EZH2low expression (p=0.001). EZH2high expression in OL epithelium was associated with a 13-fold higher risk for developing OSCC (HR=12.8). Conclusion: EZH2 expression in oral epithelium predicts OSCC transformation of OL and correlates with the level of T-cell infiltration.
article 0 Citação(ões) na Scopus
You knew you had to be there, it had to be done: Experiences of health professionals who faced the COVID-19 pandemic in one public hospital in Spain
(2023) RODRIGUEZ-MADRID, Maria Nieves; PASTOR-MORENO, Guadalupe; ALBERT-LOPEZ, Enrique; PASTOR-VALERO, Maria
IntroductionThe COVID-19 pandemic highlighted the lack of a government contingency plan for an effective response to an unexpected health crisis. This study uses a phenomenological approach to explore the experience of healthcare professionals during the first three waves of the COVID-19 pandemic in a public health hospital in the Valencia region, Spain. It assesses the impact on their health, coping strategies, institutional support, organizational changes, quality of care, and lessons learned. MethodsWe carried out a qualitative study with semi-structured interviews with doctors and nurses from the Preventive Medicine, Emergency, and Internal Medicine Services and the Intensive Care Unit, using the Colaizzi's 7-step data analysis method. ResultsDuring the first wave, lack of information and leadership led to feelings of uncertainty, fear of infection, and transmission to family members. Continuous organizational changes and lack of material and human resources brought limited results. The lack of space to accommodate patients, along with insufficient training in treating critical patients, and the frequent moving around of healthcare workers, reduced the quality of care. Despite the high levels of emotional stress reported, no sick leave was taken; the high levels of commitment and professional vocation helped in adapting to the intense work rhythms. Healthcare professionals in the medical services and support units reported higher levels of stress, and a greater sense of neglect by their institution than their colleagues in managerial roles. Family, social support, and camaraderie at work were effective coping strategies. Health professionals showed a strong collective spirit and sense of solidarity. This helped them cope with the additional stress and workload that accompanied the pandemic. ConclusionIn the wake of this experience, they highlight the need for a contingency plan adapted to each organizational context. Such a plan should include psychological counseling and continuous training in critical patient care. Above all, it needs to take advantage of the hard-won knowledge born of the COVID-19 pandemic.