A semi-automated microscopic image analysis method for scoring Ki-67 nuclear immunostaining

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorFERNEZLIAN, S. M.
dc.contributor.authorBALDAVIRA, C. M.
dc.contributor.authorSOUZA, M. L. F. de
dc.contributor.authorFARHAT, C.
dc.contributor.authorVILHENA, A. F. de
dc.contributor.authorPEREIRA, J. C. N.
dc.contributor.authorCAMPOS, J. R. M. de
dc.contributor.authorTAKAGAKI, T.
dc.contributor.authorBALANCIN, M. L.
dc.contributor.authorAB'SABER, A. M.
dc.contributor.authorCAPELOZZI, V. L.
dc.date.accessioned2023-12-15T18:56:26Z
dc.date.available2023-12-15T18:56:26Z
dc.date.issued2023
dc.description.abstractNuclear proliferation marker MIB-1 (Ki-67) immunohistochemistry (IHC) is used to examine tumor cell proliferation. However, the diagnostic or prognostic value of the Ki-67 nuclear staining intensity and location, defined as nuclear gradient (NG), has not been assessed. This study examined the potential association between Ki-67 NG and cell cycle phases and its effect on the prognosis of pulmonary typical carcinoid (PTC) tumors. We propose a method for classifying the NG of Ki-67 during the cell cycle and compare the results between PTC, pulmonary adenocarcinoma (PAD), and breast ductal carcinoma (BDC). A literature review and objective analysis of IHC-stained paraffin sections were used to determine the Ki-67 labeling index and composed a stratification of the NG into NG1, NG2, and NG3/4 categories. A semi-automated image analysis protocol was established to determine the Ki-67 NG in PTC, PAD, and BDC. High intraobserver consistency and moderate interobserver agreement were achieved in the determination of Ki-67 NG in tumor specimens. NG1 and NG2 were lower in PTC than in PAD and BDC. Cox multivariate analysis of PTC after adjusting for age and number of metastatic lymph nodes showed that Ki-67 NG1 and NG2 significantly predicted clinical outcomes. The semi-automated method for quantification of Ki-67 nuclear immunostaining proposed in this study could become a valuable diagnostic and prognostic tool in PTC.eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexWoS
dc.description.indexScopus
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP) [2018/20403-6, 2023/02755-0]
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq) [303735/2021-0]
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brasil (CAPES) [001]
dc.identifier.citationBRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, v.56, article ID e12922, 11p, 2023
dc.identifier.doi10.1590/1414-431X2023e12922
dc.identifier.eissn1414-431X
dc.identifier.issn0100-879X
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/57528
dc.language.isoeng
dc.publisherASSOC BRAS DIVULG CIENTIFICAeng
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsopenAccesseng
dc.rights.holderCopyright ASSOC BRAS DIVULG CIENTIFICAeng
dc.subjectPulmonary typical carcinoid tumoreng
dc.subjectChromatin gradienteng
dc.subjectCell cycleeng
dc.subjectImage analysiseng
dc.subjectPrognosiseng
dc.subject.othercell-cycleeng
dc.subject.other8th editioneng
dc.subject.otherantigeneng
dc.subject.otherproliferationeng
dc.subject.othercancereng
dc.subject.otherclassificationeng
dc.subject.otherexpressioneng
dc.subject.otherproteineng
dc.subject.otherpki-67eng
dc.subject.otherki67eng
dc.subject.wosBiologyeng
dc.subject.wosMedicine, Research & Experimentaleng
dc.titleA semi-automated microscopic image analysis method for scoring Ki-67 nuclear immunostainingeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryFrança
hcfmusp.affiliation.countryisofr
hcfmusp.author.externalVILHENA, A. F. de:Inst Coracao, Dept Cirurgia Torac, Sao Paulo, SP, Brazil
hcfmusp.author.externalPEREIRA, J. C. N.:Georges Pompidou European Hosp, Dept Gen Thorac Surg, Paris, France; Int Perioperat Europrogram, Paris, France
hcfmusp.citation.scopus0
hcfmusp.contributor.author-fmusphcSANDRA DE MORAIS FERNEZLIAN
hcfmusp.contributor.author-fmusphcCAMILA MACHADO BALDAVIRA
hcfmusp.contributor.author-fmusphcMARIA LUIZA FERNEZLIAN DE SOUZA
hcfmusp.contributor.author-fmusphcCECILIA FARHAT SERRANO
hcfmusp.contributor.author-fmusphcJOSE RIBAS MILANEZ DE CAMPOS
hcfmusp.contributor.author-fmusphcTERESA YAE TAKAGAKI
hcfmusp.contributor.author-fmusphcMARCELO LUIZ BALANCIN
hcfmusp.contributor.author-fmusphcALEXANDRE MUXFELDT AB'SABER
hcfmusp.contributor.author-fmusphcVERA LUIZA CAPELOZZI
hcfmusp.description.articlenumbere12922
hcfmusp.description.volume56
hcfmusp.origemWOS
hcfmusp.origem.pubmed37970922
hcfmusp.origem.scopus2-s2.0-85176910112
hcfmusp.origem.wosWOS:001104806200001
hcfmusp.publisher.cityRIBEIRAO PRETOeng
hcfmusp.publisher.countryBRAZILeng
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