Chlorhexidine susceptibility and <i>Eagle effect</i> in planktonic cells and biofilm of nosocomial isolates

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMARCHI, Ana Paula
dc.contributor.authorCORTES, Marina Farrel
dc.contributor.authorNOGUERA, Saidy Vasconez
dc.contributor.authorROSSI, Flavia
dc.contributor.authorLEVIN, Anna Sara
dc.contributor.authorCOSTA, Silvia Figueiredo
dc.contributor.authorNETO, Lauro Vieira Perdigao
dc.date.accessioned2024-02-15T14:47:47Z
dc.date.available2024-02-15T14:47:47Z
dc.date.issued2023
dc.description.abstractThe aim of this study is to evaluate the chlorhexidine gluconate (CHG) susceptibility in both planktonic cells and biofilm of 32 Gram-negative (Gn) and 6 Gram-positive (Gp) isolates by minimal inhibitory concentration (2-256 mu g/mL for Gn and 2-32 mu g/mL for Gp), minimal bactericidal concentration (4-256 mu g/mL for Gn and 2-32 mu g/mL for Gp) in planktonic cells, and minimal biofilm elimination concentration (128 >= 16,384 mu g/mL in Gn and 32 >= 16,384 mu g/mL in Gp) in biofilm environment. Our study showed that Gn isolates have higher minimal concentrations than Gp and bacteria in biofilms are more tolerant than planktonic ones. No correlation between MBC or MBEC and biofilm formation was statistically confirmed. The Eagle effect, previously described for antimicrobials and antifungals, was evidenced in this work for CHG, an antiseptic. Besides that, the phenomenon was described in 23/38 isolates (60.5%), raising minimal concentration up to >= 16,384 mu g/mL. Our study showed that clinical isolates have a high ability to form biofilm allowing them to tolerate CHG concentrations as high as the ones used in clinical practice. Therefore, attention should be given to the occurrence of this phenomenon to avoid false susceptibility results.eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexWoS
dc.description.indexScopus
dc.description.sponsorshipCAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
dc.identifier.citationEUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, v.42, n.6, p.787-792, 2023
dc.identifier.doi10.1007/s10096-023-04594-w
dc.identifier.eissn1435-4373
dc.identifier.issn0934-9723
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/57951
dc.language.isoeng
dc.publisherSPRINGEReng
dc.relation.ispartofEuropean Journal of Clinical Microbiology & Infectious Diseases
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright SPRINGEReng
dc.subjectChlorhexidineeng
dc.subjectBiofilmeng
dc.subjectMinimal inhibitory concentrationeng
dc.subjectResistanceeng
dc.subjectEagle effecteng
dc.subject.wosInfectious Diseaseseng
dc.subject.wosMicrobiologyeng
dc.titleChlorhexidine susceptibility and <i>Eagle effect</i> in planktonic cells and biofilm of nosocomial isolateseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.citation.scopus1
hcfmusp.contributor.author-fmusphcANA PAULA MARCHI ROSIN
hcfmusp.contributor.author-fmusphcMARINA FARREL CORTES
hcfmusp.contributor.author-fmusphcSAIDY LICETH VASCONEZ NOGUERA
hcfmusp.contributor.author-fmusphcFLAVIA ROSSI
hcfmusp.contributor.author-fmusphcANNA SARA SHAFFERMAN LEVIN
hcfmusp.contributor.author-fmusphcSILVIA FIGUEIREDO COSTA
hcfmusp.contributor.author-fmusphcLAURO VIEIRA PERDIGAO NETO
hcfmusp.description.beginpage787
hcfmusp.description.endpage792
hcfmusp.description.issue6
hcfmusp.description.volume42
hcfmusp.origemWOS
hcfmusp.origem.pubmed37086303
hcfmusp.origem.scopus2-s2.0-85153211280
hcfmusp.origem.wosWOS:000973353800001
hcfmusp.publisher.cityNEW YORKeng
hcfmusp.publisher.countryUNITED STATESeng
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hcfmusp.scopus.lastupdate2024-05-17
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