Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health?

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art_FERNANDES_Toxicity_of_spike_fragments_SARSCoV2_S_protein_for_2022.PDF (3.69 MB)
Citações na Scopus
19
Tipo de produção
article
Data de publicação
2022
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER
Autores
FEITOSA, Natalia Martins
BARBOSA, Ana Paula
BOMFIM, Camila Gasque
GARNIQUE, Anali M. B.
ROSA, Ivana F.
RODRIGUES, Maira S.
DORETTO, Lucas B.
COSTA, Daniel F.
CAMARGO-DOS-SANTOS, Bruno
Citação
SCIENCE OF THE TOTAL ENVIRONMENT, v.813, article ID 152345, 12p, 2022
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Despite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. In this context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Danio rerio, zebrafish. We demonstrated, for the first time, that zebrafish injected with fragment 16 to 165 (rSpike), which corresponds to the N-terminal portion of the protein, presented mortalities and adverse effects on liver, kidney, ovary and brain tissues. The conserved genetic homology between zebrafish and humans might be one of the reasons for the intense toxic effects followed inflammatory reaction from the immune system of zebrafish to rSpike which provoked damage to organs in a similar pattern as happen in severe cases of COVID-19 in humans, and, resulted in 78,6% of survival rate in female adults during the first seven days. The application of spike protein in zebrafish was highly toxic that is suitable for future studies to gather valuable information about ecotoxicological impacts, as well as vaccine responses and therapeutic approaches in human medicine. Therefore, besides representing an important tool to assess the harmful effects of SARS-CoV-2 in the aquatic environment, we present the zebrafish as an animal model for translational COVID-19 research.
Palavras-chave
Coronavirus, Danio rerio, Environmental impacts, Infection diseases, Acute respiratory syndrome
URI
https://observatorio.fm.usp.br/handle/OPI/44672
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Coleções
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - COVID-19
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - LIM/42
Artigos e Materiais de Revistas Científicas - LIM/43
Artigos e Materiais de Revistas Científicas - ODS/03
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