The Exometabolome of <i>Xylella fastidiosa</i> in Contact with <i>Paraburkholderia phytofirmans</i> Supernatant Reveals Changes in Nicotinamide, Amino Acids, Biotin, and Plant Hormones

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorFEITOSA-JUNIOR, Oseias R.
dc.contributor.authorLUBBE, Andrea
dc.contributor.authorKOSINA, Suzanne M.
dc.contributor.authorMARTINS-JUNIOR, Joaquim
dc.contributor.authorBARBOSA, Deibs
dc.contributor.authorBACCARI, Clelia
dc.contributor.authorZAINI, Paulo A.
dc.contributor.authorBOWEN, Benjamin P.
dc.contributor.authorNORTHEN, Trent R.
dc.contributor.authorLINDOW, Steven E.
dc.contributor.authorSILVA, Aline M. da
dc.date.accessioned2024-04-05T19:46:27Z
dc.date.available2024-04-05T19:46:27Z
dc.date.issued2024
dc.description.abstractMicrobial competition within plant tissues affects invading pathogens' fitness. Metabolomics is a great tool for studying their biochemical interactions by identifying accumulated metabolites. Xylella fastidiosa, a Gram-negative bacterium causing Pierce's disease (PD) in grapevines, secretes various virulence factors including cell wall-degrading enzymes, adhesion proteins, and quorum-sensing molecules. These factors, along with outer membrane vesicles, contribute to its pathogenicity. Previous studies demonstrated that co-inoculating X. fastidiosa with the Paraburkholderia phytofirmans strain PsJN suppressed PD symptoms. Here, we further investigated the interaction between the phytopathogen and the endophyte by analyzing the exometabolome of wild-type X. fastidiosa and a diffusible signaling factor (DSF) mutant lacking quorum sensing, cultivated with 20% P. phytofirmans spent media. Liquid chromatography-mass spectrometry (LC-MS) and the Method for Metabolite Annotation and Gene Integration (MAGI) were used to detect and map metabolites to genomes, revealing a total of 121 metabolites, of which 25 were further investigated. These metabolites potentially relate to host adaptation, virulence, and pathogenicity. Notably, this study presents the first comprehensive profile of X. fastidiosa in the presence of a P. phytofirmans spent media. The results highlight that P. phytofirmans and the absence of functional quorum sensing affect the ratios of glutamine to glutamate (Gln:Glu) in X. fastidiosa. Additionally, two compounds with plant metabolism and growth properties, 2-aminoisobutyric acid and gibberellic acid, were downregulated when X. fastidiosa interacted with P. phytofirmans. These findings suggest that P. phytofirmans-mediated disease suppression involves modulation of the exometabolome of X. fastidiosa, impacting plant immunity.eng
dc.description.indexPubMed
dc.description.indexScopus
dc.description.indexDimensions
dc.description.indexWoS
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP) [08/11703-4]
dc.description.sponsorshipCoordination for the Improvement of Higher Education Personnel(CAPES) [88887.285065/2018-00, 88887.318125/2019-00]
dc.description.sponsorshipProgram Sandwich-Doctorate Abroad (PDSE) [99999.009713/2014-00]
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq)
dc.description.sponsorshipOffice of Science, Office of Biological and Environmental Research, of the US Department of Energy [DE-SC0012627]
dc.description.sponsorshipU.S. Department of Energy Joint Genome Institute
dc.description.sponsorshipOffice of Science of the U.S. Department of Energy [DE-AC02-05CH11231]
dc.identifier.citationMETABOLITES, v.14, n.2, article ID 82, 26p, 2024
dc.identifier.doi10.3390/metabo14020082
dc.identifier.eissn2218-1989
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/59296
dc.language.isoeng
dc.publisherMDPIeng
dc.relation.ispartofMetabolites
dc.rightsopenAccesseng
dc.rights.holderCopyright MDPIeng
dc.subjectXylella fastidiosaeng
dc.subjectParaburkholderia phytofirmanseng
dc.subjectmetabolomicseng
dc.subjectphytopathogeneng
dc.subjectliquid chromatography-mass spectrometryeng
dc.subjectMAGIeng
dc.subject.othergibberellin biosynthesiseng
dc.subject.othermetabolic footprinteng
dc.subject.otheraminobutyric-acideng
dc.subject.otherreverse-ecologyeng
dc.subject.othercolonizationeng
dc.subject.otherbacteriaeng
dc.subject.otherinsectseng
dc.subject.otherstraineng
dc.subject.othertooleng
dc.subject.otherresistanceeng
dc.subject.wosBiochemistry & Molecular Biologyeng
dc.titleThe Exometabolome of <i>Xylella fastidiosa</i> in Contact with <i>Paraburkholderia phytofirmans</i> Supernatant Reveals Changes in Nicotinamide, Amino Acids, Biotin, and Plant Hormoneseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryAlemanha
hcfmusp.affiliation.countryisode
hcfmusp.affiliation.countryisous
hcfmusp.author.externalFEITOSA-JUNIOR, Oseias R.:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508900 Sao Paulo, SP, Brazil; DOE Joint Genome Inst, Berkeley, CA 94720 USA; Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA; Ludwig Maximilian Univ Munich, Genet, Bioctr, D-82152 Munich, Germany
hcfmusp.author.externalLUBBE, Andrea:Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USA; Bioact Co, Brightseed, San Francisco, CA 94080 USA
hcfmusp.author.externalKOSINA, Suzanne M.:Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USA
hcfmusp.author.externalMARTINS-JUNIOR, Joaquim:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508900 Sao Paulo, SP, Brazil; Natl Ctr Energy & Mat Res CNPEN, Natl Biorenewables Lab, BR-13083100 Campinas, SP, Brazil
hcfmusp.author.externalBACCARI, Clelia:Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
hcfmusp.author.externalZAINI, Paulo A.:Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
hcfmusp.author.externalBOWEN, Benjamin P.:DOE Joint Genome Inst, Berkeley, CA 94720 USA; Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USA
hcfmusp.author.externalNORTHEN, Trent R.:DOE Joint Genome Inst, Berkeley, CA 94720 USA; Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USA
hcfmusp.author.externalLINDOW, Steven E.:Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
hcfmusp.author.externalSILVA, Aline M. da:Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508900 Sao Paulo, SP, Brazil
hcfmusp.citation.scopus0
hcfmusp.contributor.author-fmusphcDEIBS BARBOSA
hcfmusp.description.articlenumber82
hcfmusp.description.issue2
hcfmusp.description.volume14
hcfmusp.origemWOS
hcfmusp.origem.dimensionspub.1168251191
hcfmusp.origem.pubmed38392974
hcfmusp.origem.scopus2-s2.0-85185658500
hcfmusp.origem.wosWOS:001174021100001
hcfmusp.publisher.cityBASELeng
hcfmusp.publisher.countrySWITZERLANDeng
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