Epidemiologic case investigation on the zoonotic transmission of Methicillin-resistant Staphylococcus pseudintermedius among dogs and their owners

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorGUIMARAES, Luciana
dc.contributor.authorTEIXEIRA, Izabel Mello
dc.contributor.authorSILVA, Isabella Thomaz da
dc.contributor.authorANTUNES, Milena
dc.contributor.authorPESSET, Camilla
dc.contributor.authorFONSECA, Carolina
dc.contributor.authorSANTOS, Ana Luiza
dc.contributor.authorCORTES, Marina Farrel
dc.contributor.authorPENNA, Bruno
dc.date.accessioned2024-04-05T19:36:37Z
dc.date.available2024-04-05T19:36:37Z
dc.date.issued2023
dc.description.abstractDogs often carry methicillin-resistant Staphylococci asymptomatically. These bacteria are frequently linked to conditions such as canine pyoderma and otitis. Close interaction between dogs and humans can facilitate the exchange of resistant strains, particularly Methicillin-resistant Staphylococcus pseudintermedius (MRSP). This represents a public health issue, since these strains, in addition to occasionally causing infections in humans, can also serve as a source of resistance and virulence genes for strains of greater importance in human medicine, such as Staphylococcus aureus. Furthermore, MRSP strains are often multidrug resistant, which ends up compromising the treatment of infections. This study aimed to assess the potential transmission of Staphylococcus pseudintermedius among dogs and their owners. We examined a total of one hundred canine samples collected from cases of pyoderma and otitis to detect the presence of staphylococci. Simultaneously, we conducted evaluations on all dog owners. Staphylococci strains were identified using MALDI-TOF MS and PCR targeting the nuc gene. Methicillin resistance screening was also performed by detecting the mecA gene using PCR. Among the sampled dogs, 64 carried S. pseudintermedius. Nine were identified as MRSP. In six instances, dogs and their owners exhibited S. pseudintermedius. These samples underwent genome sequencing and were screened for antimicrobial resistance genes, SCCmec typing, MLST characterization, and Single Nucleotide Polymorphisms (SNP) analyses. The results of the phylogenetic analysis revealed that in three cases, dogs and owners had closely related isolates, suggesting interspecies transmission. Two of these cases involved MRSP and one MSSP. Moreover, in the two MRSP cases, the same SCCmec type (type V) was detected. Additionally, the sequence type was consistent across all three cases involving dogs and owners (MSSP ST2277, MRSP ST2282, and ST2286). These findings strongly indicate a transmission event. Since Staphylococcus pseudintermedius is primarily isolated from canine samples, it is plausible that dogs may have acted as a potential source. In the remaining three cases, despite identifying the same species in both samples, they had notable phylogenetic differences. (c) 2023 The Author(s).eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexScopus
dc.description.indexDimensions
dc.description.indexWoS
dc.description.sponsorshipFundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro -FAPERJ [E26/211.554/2019, E-26/201.328/2021]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico -CNPq [406057/2016-8, 443764/2018-2]
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior -CAPES
dc.description.sponsorshipBill and Melinda Gates Foundation
dc.identifier.citationJOURNAL OF INFECTION AND PUBLIC HEALTH, v.16, suppl.1, p.183-189, 2023
dc.identifier.doi10.1016/j.jiph.2023.10.041
dc.identifier.eissn1876-035X
dc.identifier.issn1876-0341
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/59110
dc.language.isoeng
dc.publisherELSEVIER SCIENCE LONDONeng
dc.relation.ispartofJournal of Infection and Public Health
dc.rightsopenAccesseng
dc.rights.holderCopyright ELSEVIER SCIENCE LONDONeng
dc.subjectStaphylococcus pseudintermediuseng
dc.subjectMRSPeng
dc.subjectZoonotic transmissioneng
dc.subject.othersmall animalseng
dc.subject.otherinfectioneng
dc.subject.otherhumanseng
dc.subject.othercolonizationeng
dc.subject.wosPublic, Environmental & Occupational Healtheng
dc.subject.wosInfectious Diseaseseng
dc.titleEpidemiologic case investigation on the zoonotic transmission of Methicillin-resistant Staphylococcus pseudintermedius among dogs and their ownerseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalGUIMARAES, Luciana:Univ Fed Fluminense, Inst Biomed, Lab Cocos Gram Posit, Niteroi, RJ, Brazil; Fluminense Fed Univ, Grad Program Vet Med, Niteroi, RJ, Brazil
hcfmusp.author.externalTEIXEIRA, Izabel Mello:Univ Fed Fluminense, Inst Biomed, Lab Cocos Gram Posit, Niteroi, RJ, Brazil; Univ Fed Rio de Janeiro, Inst Microbiol Paulo Goes, Grad Program Microbiol, Rio de Janeiro, Brazil
hcfmusp.author.externalSILVA, Isabella Thomaz da:Univ Fed Fluminense, Inst Biomed, Lab Cocos Gram Posit, Niteroi, RJ, Brazil; Fluminense Fed Univ, Grad Program Vet Med, Niteroi, RJ, Brazil
hcfmusp.author.externalANTUNES, Milena:Univ Fed Fluminense, Inst Biomed, Lab Cocos Gram Posit, Niteroi, RJ, Brazil; Univ Fed Rio de Janeiro, Inst Microbiol Paulo Goes, Grad Program Microbiol, Rio de Janeiro, Brazil
hcfmusp.author.externalPESSET, Camilla:Univ Fed Fluminense, Inst Biomed, Lab Cocos Gram Posit, Niteroi, RJ, Brazil
hcfmusp.author.externalFONSECA, Carolina:Univ Fed Fluminense, Inst Biomed, Lab Cocos Gram Posit, Niteroi, RJ, Brazil; Fluminense Fed Univ, Grad Program Vet Med, Niteroi, RJ, Brazil
hcfmusp.author.externalSANTOS, Ana Luiza:Univ Fed Fluminense, Inst Biomed, Lab Cocos Gram Posit, Niteroi, RJ, Brazil
hcfmusp.author.externalPENNA, Bruno:Univ Fed Fluminense, Inst Biomed, Lab Cocos Gram Posit, Niteroi, RJ, Brazil; Fluminense Fed Univ, Grad Program Vet Med, Niteroi, RJ, Brazil
hcfmusp.citation.scopus2
hcfmusp.contributor.author-fmusphcMARINA FARREL CORTES
hcfmusp.description.beginpage183
hcfmusp.description.endpage189
hcfmusp.description.issuesuppl 1
hcfmusp.description.volume16
hcfmusp.origemWOS
hcfmusp.origem.dimensionspub.1165573731
hcfmusp.origem.pubmed37973497
hcfmusp.origem.scopus2-s2.0-85177031801
hcfmusp.origem.wosWOS:001133767800024
hcfmusp.publisher.cityLONDONeng
hcfmusp.publisher.countryENGLANDeng
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