In situ expression of Th17 immunologic mediators in American cutaneous leishmaniasis caused by Leishmania (V.) braziliensis and Leishmania (L.) amazonensis in the Brazilian Amazon

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorRODRIGUES, G. F.
dc.contributor.authorALCâNTARA, L. S.
dc.contributor.authorBARROS, J. P. B.
dc.contributor.authorLIMA, A. C. S. de
dc.contributor.authorCAMPOS, M. B.
dc.contributor.authorMORAES, C.
dc.contributor.authorFERREIRA, A. F.
dc.contributor.authorMATTA, V. L. R.
dc.contributor.authorLAURENTI, M. D.
dc.contributor.authorCORBETT, C. E. P.
dc.contributor.authorSILVEIRA, F. T.
dc.contributor.authorGOMES, C. M. C.
dc.date.accessioned2024-03-13T19:57:55Z
dc.date.available2024-03-13T19:57:55Z
dc.date.issued2023
dc.description.abstractAmerican cutaneous leishmaniasis (ACL) presents a wide spectrum of clinical and immunopathological manifestations. In Brazil, Leishmania (L.) amazonensis[La] and Leishmania(V.)braziliensis[Lb] show the highest pathogenic potential for humans causing different clinical forms: localized cutaneous leishmaniasis (LCL : Lb/La), anergic diffuse cutaneous leishmaniasis (ADCL : La) and mucocutaneous leishmaniasis (MCL : Lb). ADCL and MCL are the most severe forms and infection leads to a cellular immune response at the hyposensitivity and hypersensitivity poles. Th17-cells are involved in the ACL pathogenesis, are derived from naïve TCD4+ cells regulated by RORγt, differentiate in presence of IL-6, TGF-β, IL- 1β, IL-23 and express IL-17. Aim of this study was to characterize the cellular immune response mediated by Th17-profile cells through in situ determination of the expression of RORγt, IL-17, IL-6, TGF-β, IL-1β, and IL-23 in the ACL clinical-immunopathological spectrum caused by L.(L.)amazonensis and L.(V.)braziliensis. Biopsies of skin and mucosal lesions from forty patients including ADCL(n=8), LCL[La](n=17), LCL[Lb](n=9) and MCL(n=6), were examined by immunohistochemistry. The immunostained cells density (cells/mm2) was determined in image analysis system using AxionVision 4.8 software (Zeiss). As the disease evolution time (DET) was different among ACL patients, the effect of DET on the expression of immunological markers was evaluated in different clinical forms and histopathological changes, using ANCOVA. Our results showed significantly increased expression of RORγt, IL-17, IL-6, IL-1β and IL-23 in patients with ACL polar forms (ADCL and MCL); higher TGF-β expression was found in ADCL. DET influenced the expression of RORγt and IL-6 in: clinical forms of ACL and in categories of parasitism. DET also affected the production of RORγt, IL-17, IL-6, TGF-β and IL-1β in types of inflammatory infiltrate, evidencing that DET had effect on the expression of Th17 profile cytokines in ACL. Together, the expression of immunological mediators of Th17 profile in the ACL spectrum, as well as the DET effect, demonstrate the participation of this cell lineage in the immunopathogenesis of ACL, mainly in the polar and more severe forms of ACL spectrum. The dubious role played by Th17-cells may favors immune response suppression and parasitic persistence in ADCL, while in MCL it contributes to an exacerbated immune response and parasite scarcity.eng
dc.description.indexPubMed
dc.description.indexScopus
dc.description.indexDimensions
dc.description.sponsorshipLaboratório de Patologia de Moléstias Infecciosas, (LIM - 50 HC-FMUSP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, (2014/50315-0, 2019/17957-2)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES, (888.377397/2019-01, 88887.649146/2021-00)
dc.description.sponsorshipUniversidade de São Paulo, USP
dc.identifier.citationFRONTIERS IN TROPICAL DISEASES, v.4, article ID 1067595, p, 2023
dc.identifier.doi10.3389/fitd.2023.1067595
dc.identifier.issn2673-7515
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/58641
dc.language.isoeng
dc.publisherFRONTIERS MEDIA SAeng
dc.relation.ispartofFrontiers in Tropical Diseases
dc.rightsopenAccesseng
dc.rights.holderCopyright FRONTIERS MEDIA SAeng
dc.subjectAmerican cutaneous leishmaniasiseng
dc.subjectcellular immunityeng
dc.subjectimmunohistochemistryeng
dc.subjectleishmania (V.) braziliensiseng
dc.subjectleishmania(L.)amazonensiseng
dc.subjectTh17-cellseng
dc.titleIn situ expression of Th17 immunologic mediators in American cutaneous leishmaniasis caused by Leishmania (V.) braziliensis and Leishmania (L.) amazonensis in the Brazilian Amazoneng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalBARROS, J. P. B.:Departamento de Patologia, Laboratorio de Patologia de Molestias Infecciosas, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil
hcfmusp.author.externalLIMA, A. C. S. de:Instituto Evandro Chagas, Secretaria de Ciencia, Tecnologia, Inovaçao e Insumos Estrategicos em Saude, Ministerio da Saude, Para, Ananindeua, Brazil
hcfmusp.author.externalCAMPOS, M. B.:Instituto Evandro Chagas, Secretaria de Ciencia, Tecnologia, Inovaçao e Insumos Estrategicos em Saude, Ministerio da Saude, Para, Ananindeua, Brazil
hcfmusp.author.externalMORAES, C.:Faculdade de Saude Publica da Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil
hcfmusp.author.externalSILVEIRA, F. T.:Instituto Evandro Chagas, Secretaria de Ciencia, Tecnologia, Inovaçao e Insumos Estrategicos em Saude, Ministerio da Saude, Para, Ananindeua, Brazil, Nucleo de Medicina Tropical, Universidade Federal do Para, Para, Belem, Brazil
hcfmusp.citation.scopus0
hcfmusp.contributor.author-fmusphcGABRIELA FERNANDES RODRIGUES
hcfmusp.contributor.author-fmusphcLARISSA DOS SANTOS ALCANTARA
hcfmusp.contributor.author-fmusphcAUREA FAVERO FERREIRA
hcfmusp.contributor.author-fmusphcVANIA LUCIA RIBEIRO DA MATTA
hcfmusp.contributor.author-fmusphcMARCIA DALASTRA LAURENTI
hcfmusp.contributor.author-fmusphcCARLOS EDUARDO PEREIRA CORBETT
hcfmusp.contributor.author-fmusphcCLAUDIA MARIA DE CASTRO GOMES
hcfmusp.description.articlenumber1067595
hcfmusp.description.volume4
hcfmusp.origemSCOPUS
hcfmusp.origem.dimensionspub.1155294219
hcfmusp.origem.scopus2-s2.0-85183656570
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