Diaphragmatic hernia repair porcine model to compare the performance of biodegradable membranes against Gore-Tex®

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art_SCUGLIA_Diaphragmatic_hernia_repair_porcine_model_to_compare_the_2023.PDF (3.16 MB)
Citações na Scopus
0
Tipo de produção
article
Data de publicação
2023
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
SPRINGER
Autores
SCUGLIA, Marianna
FRAZAO, Laura P.
MIRANDA, Alice
MARTINS, Albino
BARBOSA-SEQUEIRA, Joana
COIMBRA, Diana
REIS, Rui L.
NOGUEIRA-SILVA, Cristina
NEVES, Nuno M.
Citação
PEDIATRIC SURGERY INTERNATIONAL, v.40, n.1, article ID 7, 12p, 2023
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: Patch repair of congenital diaphragmatic hernia (CDH) using Gore-Tex (R) is associated with infection, adhesions, hernia recurrence, long-term musculoskeletal sequels and poor tissue regeneration. To overcome these limitations, the performance of two novel biodegradable membranes was tested to repair CDH in a growing pig model.Methods: Twelve male pigs were randomly assigned to 3 different groups of 4 animals each, determined by the type of patch used during thoracoscopic diaphragmatic hernia repair (Gore-Tex (R), polycaprolactone electrospun membrane-PCLem, and decellularized human chorion membrane-dHCM). After 7 weeks, all animals were euthanized, followed by necropsy for diaphragmatic evaluation and histological analysis.Results: Thoracoscopic defect creation and diaphragmatic repair were performed without any technical difficulty in all groups. However, hernia recurrence rate was 0% in Gore-Tex (R), 50% in PCLem and 100% in dHCM groups. At euthanasia, Gore-Tex (R) patches appeared virtually unchanged and covered with a fibrotic capsule, while PCLem and dHCM patches were replaced by either floppy connective tissue or vascularized and floppy regenerated membranous tissue, respectively.Conclusion: Gore-Tex (R) was associated with a higher survival rate and lower recurrence. Nevertheless, the proposed biodegradable membranes were associated with better tissue integration when compared with Gore-Tex (R).
Palavras-chave
Gore-Tex (R), Electrospun fibrous mesh, Decellularized human chorion membrane, Congenital diaphragmatic hernia repair, Thoracoscopic surgery
URI
https://observatorio.fm.usp.br/handle/OPI/57592
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Coleções
Artigos e Materiais de Revistas Científicas - FM/Outros
Artigos e Materiais de Revistas Científicas - LIM/14