Evaluation of IFN-gamma secretion after stimulation with C. neoformans and C. gattii antigens in individuals with frequent exposure to the fungus

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0
Tipo de produção
article
Data de publicação
2022
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
MASSON EDITEUR
Autores
MACIEL, Isabel Feitosa
FREITAS-XAVIER, Roseli Santos de
VICENTINI, Adriana Pardini
APOLIANO, Carlos Fernando
FERNANDES, Juliana Ruiz
DIAS, Alana dos Santos
GIMENES, Viviane Favero Mazo
BENARD, Gil
Citação
JOURNAL DE MYCOLOGIE MEDICALE, v.32, n.2, article ID 101230, 5p, 2022
Projetos de Pesquisa
Unidades Organizacionais
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Resumo
In this study we produced antigenic extracts from prototypical strains of C. neoformans (VNI-VNIV) and C. gattii (VGI-VGIV) and tested IFN-gamma secretion by Elispot. Antigens from the eight Cryptococcus molecular types (VNI -VNIV and VGI - VGIV) were obtained after capsule reduction. IFN-gamma secretion by Elispot method were stimulated with C. neoformans and C. gattii antigens. Peripheral blood mononuclear cells of fourteen healthy control subjects, being: five ecotourists, two mycologists, three poultry keepers, and four individuals without reports of exposure to the fungus. We observed a significant increase in IFN-gamma secretion in the group of ecotourists, mycologists and bird keepers in relation to the group of individuals without reports of occupational exposures to these agents. Our results suggest the significant increase in IFN-gamma secretion may be related to the continuous exposure of these groups of individuals to the fungus, as well as to the specific antigen memory immune response developed during exposure to Cryptococcus.
Palavras-chave
Cryptococcus neoformans, Cryptococcus gattii, IFN-gamma
URI
https://observatorio.fm.usp.br/handle/OPI/47096
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MDT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/31
Artigos e Materiais de Revistas Científicas - LIM/36
Artigos e Materiais de Revistas Científicas - LIM/53
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