One-year surveillance of SARS-CoV-2 in wastewater from vulnerable urban communities in metropolitan Sao Paulo, Brazil

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art_BARBOSA_Oneyear_surveillance_of_SARSCoV2_in_wastewater_from_vulnerable_2022.PDF (998.19 KB)
Citações na Scopus
13
Tipo de produção
article
Data de publicação
2022
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
IWA PUBLISHING
Autores
BARBOSA, Mikaela Renata Funada
GARCIA, Suzi Cristina
BRUNI, Antonio de Castro
MACHADO, Flavio Silva
OLIVEIRA, Roberto Xavier de
DROPA, Milena
LEAL, Elcio
BRANDAO, Carlos Jesus
SILVA, Renan Lourenco Oliveira da
Citação
JOURNAL OF WATER AND HEALTH, v.20, n.2, p.471-490, 2022
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
The current COVID-19 pandemic has emphasized the vulnerability of communities living in the urban outskirts and informal settlements. The lack of reliable COVID-19 case data highlights the importance and application of wastewater-based epidemiology. This study aimed to monitor the COVID-19 trends in four vulnerable urban communities (slums and low-income neighborhoods) in metropolitan SAo Paulo by assessing the SARS-CoV-2 RNA viral load in wastewater. We analyzed 160 samples from May 2020 to June 2021 with weekly or fortnightly samplings. The samples were ultracentrifuged with glycine elution and quantified by N1/N2 SARS-CoV-2 RT-qPCR. The results of positivity were 100% (Paraisopolis, Heliopolis and Cidade Tiradentes) and 76.9% (Vila Brasilandia). The new case numbers of COVID-19, counted from the onset of symptoms, positively correlated with SARS-CoV-2 N1 viral loads from the two largest communities (p<0.001). SARS-CoV-2 infectivity was tested in Vero E6 cells after concentration with the two techniques, ultrafiltration (Centricon((R)) Plus-70 10 kDa) and sucrose cushion ultracentrifugation, but none of the evaluated samples presented positive results. Next-generation sequencing (NGS) analysis from samples collected in March and August 2021 revealed the presence of the clade 20 J (lineage P.1) belonging to the most prevalent circulating variant in the country. Our results showed that wastewater surveillance data can be used as complementary indicators to monitor the dynamics and temporal trends of COVID-19. The infectivity test results strengthened the evidence of low risk of infection associated with SARS-CoV-2 in wastewater.
Palavras-chave
environmental surveillance, infectivity, SARS-CoV-2, sequencing, vulnerable communities, wastewater
URI
https://observatorio.fm.usp.br/handle/OPI/50743
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Coleções
Artigos e Materiais de Revistas Científicas - FM/Outros
Artigos e Materiais de Revistas Científicas - COVID-19
Artigos e Materiais de Revistas Científicas - LIM/52
Artigos e Materiais de Revistas Científicas - ODS/03
Artigos e Materiais de Revistas Científicas - ODS/06
Artigos e Materiais de Revistas Científicas - ODS/11